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Bio Lab 111 Homework Assignment

Bio Lab 111 Homework Assignment - 300 base PV92 transposon...

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November 4, 2010 Bio Lab 111 Homework Assignment 7. a) Regulatory Sequence – yes. Regulatory sequences are critical for allowing cells to produce the appropriate proteins. b) Exon – Yes, the insertion of Alu-PV92 in an exon will be produced and/or functional because an exon is a coding region in a DNA. The A, T, G, and C within exons code for the amino acids that make up the functional protein. c ) Intron – No, the insertion of a Alu-PV92, found within an intron, will not be able to produce or have a functional protein. An intron is a non-coding region therefore the insertion has no effect on the production of the gene’s protein. 10. Gel electrophoresis purpose is to amplify a segment in the DNA to determine the presence of the
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Unformatted text preview: 300 base PV92 transposon. This is accomplished by putting DNA samples onto a gel which then is applied an electric current. Because, DNA is negatively charged, it migrates through the gel toward the positive electrode. Shorter molecules migrate faster than longer ones. 14. The pattern on the gel would be a ladder of different strands indicating heterozygous. The offspring DNA would then be a heterozygous with PV92. The offspring potentially could pass PV92 down to their children because the offspring will carry the dominant trait from the mother....
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