Chemistry 30A3 Fall 2011 Labs Dr. Troendle 53

Chemistry 30A3 Fall 2011 Labs Dr. Troendle 53 - 2 CF 3 OH =...

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Chemical Biology 3OA3, September 2009 Page 53 3. O BJECTIVES In this lab, we will examine orange peel esterase's (OPE's) regioselectivity against a series of diester substrates. You will be organized into groups by your TA, and each group member will synthesize a unique diester compound to test as an esterase substrate. You will then determine both the site and rate of esterase activity. By combining the results of your activity assays, your group will devise a model of the esterase active site that explains the activity you observe. Week 1 O Cl O O OH R 3 O O O O R 3 R 4 R 4 R 3 = Me, Et or CH
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Unformatted text preview: 2 CF 3 OH = meta or para R 4 = Me, Et, C 9 H 19 , Ph or t-Bu Week 2 O O O O R 4 R 3 O O H O O R 4 O O OH R 3 (1) Site of cleavage? (2) Rate of cleavage? &/or Orange Peel Esterase 4. S AFETY N OTES Many acid chlorides are lachrymators: they induce tears. Keep the acid chlorides in the hood, and in CAPPED vials while weighing. Keep all containers closed wherever possible: this will avoid vapourization, and also prevent spontaneous hydrolysis of the acid chloride by moisture in the air. Wear gloves when handling these compounds....
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This note was uploaded on 01/08/2012 for the course CHM 30A3 taught by Professor Ricktroendle during the Fall '11 term at UNF.

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