Chemical Biology 3OA3, September 2009 Page 63 A PPENDIX 1: M ICROSCALE L ABORATORY T ECHNIQUES By W. J. Leigh Traditionally, experiments in organic chemistry are carried out on a macroscale level, employing quantities of chemicals on the order of 5-100 g, using glassware designed to contain between 25 and 500 mL of liquids. For quantities of materials in the 0.005-0.5 gram range, one employs different, " microscale " techniques and equipment in order to carry out the various standard organic laboratory operations. In the following, the student is introduced to the special equipment used in microscale experiments, as well as the somewhat different methods which are used. Basic Equipment The glassware used for microscale experiments is contained in a kit. Some of the contents of the microscale kit are illustrated in the drawing below. The conical vial is used as a reaction vessel, for extractions, and as a storage container. Its flat base allows it to stand upright on the laboratory bench. The
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