Chemical Biology 3OA3, September 2009 Page 64 3 . Push the plunger down to the first detent position. Do not press the plunger to the second position. (If the plunger is pressed to the second detent, an incorrect volume of liquid will be delivered). 4 . Dip the tip of the pipette into the liquid sample. Do not immerse the entire length of the plastic tip in the liquid. It is best to dip the tip only to a depth of about one centimetre. 5 . Release the plunger slowly . Do not allow the plunger to snap back, or liquid may splash up into the plunger mechanism and ruin the pipette. Furthermore, rapid release of the plunger may cause air bubbles to be drawn into the pipette. At this point the pipette has been filled. 6 . Move the pipette to the receiving vessel. Touch the tip of the pipette to an interior wall of the container, and slowly push the plunger down to the first detent. This action will dispense the liquid into the container. 7
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This note was uploaded on 01/08/2012 for the course CHM 30A3 taught by Professor Ricktroendle during the Fall '11 term at UNF.