Hum_Mol_Genet_Lecture_4_February_7_2012

Hum_Mol_Genet_Lecture_4_February_7_2012 - Recombinant DNA...

Info iconThis preview shows pages 1–21. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Recombinant DNA Technology Site directed mutagenesis Genetics vs. Reverse Genetics Gene expression in bacteria and viruses Gene expression in yeast Genetic engineering in plants Genetic engineering in animals Gene therapy in mammals Detection of disease alleles Recombinant DNA Technology Site directed mutagenesis Base pair substitution Insertion Deletion Plasmid or PCR based approach Genetics Genetics Mutant phenotype Mutant allele DNA sequence Protein sequence Reverse Genetics Reverse Genetics Protein sequence DNA sequence Mutant allele Mutant phenotype Recombinant DNA Technology Restriction fragment length polymorphism Restriction digest Gel or gel + Southern blot Identify linkage to the mutant allele Recombinant DNA Technology Gene disruption Gene knockout Insert selectable marker into gene Recombinant DNA Technology Two-step expression vector Chromosomal lac inducible T7 RNA pol T7 RNA pol initiates expression of plasmid Recombinant DNA Technology Bacterial expression of hGH No human signal sequence Add bacterial signal sequence Recombinant DNA Technology...
View Full Document

Page1 / 90

Hum_Mol_Genet_Lecture_4_February_7_2012 - Recombinant DNA...

This preview shows document pages 1 - 21. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online