Unformatted text preview: OK, now you have all of your peak areas.
Let’s assume you knew what you were doing
and all the areas were measured properly. You either assume that response is linear over
the entire concentration range or measure it.
concunknown = Areaunknown
Areaknown concknown This is assuming that the same injection
volume was used for both the unknown and
standard. Now, determine the concentration of X in you
concunknown = 3830
0.200 µg / µl
2000 = 0.384 µg / µl
You can now convert to a more appropriate
concentration if required. ...
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