24_Chromatography-page15 - OK now you have all of your peak...

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Unformatted text preview: OK, now you have all of your peak areas. Let’s assume you knew what you were doing and all the areas were measured properly. You either assume that response is linear over the entire concentration range or measure it. Then: concunknown = Areaunknown Areaknown concknown This is assuming that the same injection volume was used for both the unknown and standard. Now, determine the concentration of X in you unknown. Areaunknown concknown concunknown = Areaknown concunknown = 3830 0.200 µg / µl 2000 = 0.384 µg / µl You can now convert to a more appropriate concentration if required. ...
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