26_LC-page7 - Starting solvent should have a polarity that...

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Unformatted text preview: Starting solvent should have a polarity that adequately resolves the first few components. Final polarity should adequately resolve the last few species in a timely manner. Now play connect the polarity - attempt various blending rates to separate the remaining components. c a b f d Unlike GC equipment, many HPLC systems have a modular design - can simply add a new ‘box’ to change/extent capabilities. e a - gradient controller b - pump/dampening system c - sample introduction d - column/pre-column e - detector f - data output All solvents should be ‘HPLC’ grade. This is a type of reagent grade material. It has been filtered using a 0.2 µm filter. You can purchase it or produce it yourself. Filtered solvent helps extend pump life by preventing scoring. It also reduces the chances of a column plugging. There is also a wider range of how to do things like produce a flow or gradient. We’ll cover some of the basic approaches. All solvents should be degassed prior to use. This reduces the chances of bubbles being formed in the column or detector. Oxygen present at high pressure can also cause a problem. Methods that can be used Displacement with a less soluble gas Applying a vacuum Heating the solvent. ...
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