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Unformatted text preview: Starting solvent should have a polarity that
adequately resolves the first few
Final polarity should adequately resolve
the last few species in a timely manner.
Now play connect the polarity - attempt
various blending rates to separate the
remaining components. c
a b f d Unlike GC equipment, many HPLC
systems have a modular design - can
simply add a new ‘box’ to change/extent
capabilities. e a - gradient controller
b - pump/dampening system
c - sample introduction d - column/pre-column
e - detector
f - data output All solvents should be ‘HPLC’ grade.
This is a type of reagent grade material.
It has been filtered using a 0.2 µm filter.
You can purchase it or produce it yourself.
Filtered solvent helps extend pump life by
preventing scoring. It also reduces the
chances of a column plugging. There is also a wider range of how to do
things like produce a flow or gradient.
We’ll cover some of the basic approaches. All solvents should be degassed prior to use.
This reduces the chances of bubbles being
formed in the column or detector. Oxygen
present at high pressure can also cause a
Methods that can be used
Displacement with a less soluble gas
Applying a vacuum
Heating the solvent. ...
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- Fall '08