24_Chromatography-page2

24_Chromatography-page2 - A solute partitions between two...

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Unformatted text preview: A solute partitions between two phases (equilibrium). Separation is based on relative retention. Making the column longer will increase the degree of separation. In plate theory, we treat our chromatographic column as though it was a ‘static’ system in equilibrium. Each species exhibits an equilibrium between the mobile and stationary phase. Amobile phase A stationary phase For a separation, assume that: The column is of fixed length. K= Flow is held constant. We can then describe several terms based on how our average sample species performs. The first thing to do is measure how long it takes for our solute to travel through our column. ...
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