24_Chromatography-page2 - A solute partitions between two...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: A solute partitions between two phases (equilibrium). Separation is based on relative retention. Making the column longer will increase the degree of separation. In plate theory, we treat our chromatographic column as though it was a ‘static’ system in equilibrium. Each species exhibits an equilibrium between the mobile and stationary phase. Amobile phase A stationary phase For a separation, assume that: The column is of fixed length. K= Flow is held constant. We can then describe several terms based on how our average sample species performs. The first thing to do is measure how long it takes for our solute to travel through our column. ...
View Full Document

{[ snackBarMessage ]}

Ask a homework question - tutors are online