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Unformatted text preview: A solute partitions
between two phases
Separation is based on
Making the column
longer will increase the
degree of separation. In plate theory, we treat our
chromatographic column as though it
was a ‘static’ system in equilibrium.
Each species exhibits an equilibrium
between the mobile and stationary
Amobile phase A stationary phase For a separation, assume that:
The column is of fixed length.
K= Flow is held constant.
We can then describe several terms based on
how our average sample species performs.
The first thing to do is measure how long it takes
for our solute to travel through our column. ...
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