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24_Chromatography-page5

24_Chromatography-page5 - The number of plates can be...

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t R injection width The number of plates can be determined from the retention time and peak width. It doesn’t matter what units are used as long as they are the same. The number of plates is then calculated as: N = 16 This approach is taken because peaks evolve as Gaussian-like shapes and can be treated statistically. In essence, we are taking + 2 " . t R W ( ) 2 N = 1000 N = 100 In this example, we have materials with the same elution time but different numbers of plates. It is often difficult to accurately measure peak width. ! The peak may co-elute with another.
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