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24_Chromatography-page11

24_Chromatography-page11 - tR tm tR Retention volume or...

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t R t’ R t m Retention volume or time may be used for identification. For a homologous series, V R can be accurately determined by: ln V’ n = a + bn where V’ n = adjusted retention volume n = carbon number a, b = fit parameters and V’ n = V n - V m To determine an unknown carbon number: x = n 1 + (n 2 -n 1 ) n 2 > x > n 1 This can only be used for straight chain
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