24_Chromatography-page9

24_Chromatography-page9 - slowest An understanding of the...

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Unformatted text preview: slowest An understanding of the effects of each term will help you design/select appropriate columns and optimum flows. fastest You don’t need to calculate H for each column/eluent combination to be able to use this relationship. Once the column is packed, nothing can be done to reduce the A term. Its effect can be reduced by using regular sized packing small diameter packing not allowing any loose packing or dead space in the column The effect of the B term is flow dependent. As you increase the flow, the time for diffusion is reduced. You should keep the flow as high as possible within the limits imposed by the instrument and the C term. trailing tail broadening ...
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This note was uploaded on 01/07/2012 for the course CHEM 290 taught by Professor Harvey during the Fall '08 term at SUNY Stony Brook.

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