Unformatted text preview: slowest An understanding of the effects of each
term will help you design/select
appropriate columns and optimum flows. fastest You don’t need to calculate H for each
column/eluent combination to be able to
use this relationship. Once the column is packed, nothing can be done
to reduce the A term.
Its effect can be reduced by using
regular sized packing
small diameter packing
not allowing any loose packing or
dead space in the column The effect of the B term is flow dependent.
As you increase the flow, the time for
diffusion is reduced.
You should keep the flow as high as
possible within the limits imposed by the
instrument and the C term. trailing tail broadening ...
View Full Document