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24_Chromatography-page10 - You can minimize the effect of...

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You can minimize the effect of the C term by: Using “thin” coatings of the stationary phase on a solid support. Use less viscous phases. Keep the flow as low as possible - limited by the effect of the B term. The best velocity is a function of the Van deemter equation and practical conditions. You need to have a useable analysis time. Also, since the effects of B are greater than C, it is best to set the flow a little on the high side in case it changes slightly during the analysis. H u A B/u Cu u opt This plot is for a typical packed column GC analysis. Gases have high diffusions and
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