shphosphate_04 - On a separate sheet graph the standard...

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APPENDIX FOR PROJECT #5 PHOSPHATE Name: _________________________ Date of Experiment: _________________________ Group Members : ______________ ______________ ______________ Sample Description Sample I.D. Description Date Collected or Prepared Lake Osceola, Inlet Lake Osceola, Outlet 0.1 mg/L Control DATA Absorption Spectrum Wavelength (nm) Absorbance Transmittance 775 800 825 850 875 900 925 Comments Concerning Sample Used: DATA (continued)
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Preparation of Standards and Control Sample Description Conc. of Stock (mg/l) Vol. Stock Used (ml) Final Vol. of standard/control (ml) 0 mg/L 0.01 mg/L 0.025 mg/L 0.10 mg/L 0.25 mg/L 0.50 mg/L Comments Concerning Preparation of Standards: Spectrophotometer Readings Sample Description Absorbance Transmittance 0 mg/L 0.01 mg/L 0.025 mg/L 0.10 mg/L 0.25 mg/L 0.50 mg/L Comments Concerning Spectrophotometer Readings:
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DATA ANALYSIS On a separate sheet, plot the absorption spectrum which is a plot of absorbance (y-axis) versus wavelength (x-axis). Clearly label the optimum wavelength for analysis on the plot.
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Unformatted text preview: On a separate sheet, graph the standard solution absorbance (x-axis) vs. concentration (y-axis). Calculate and plot (on the same graph) the regression line using the linear method of least squares. Determine the R 2 value (goodness of fit parameter) of the regression curve. Show work for the regression line and R 2 value on a separate sheet. Best-fit line Concentration, mg/L = ______________ Abs + _____________ R 2 = ______________ Determine the concentration of the environmental samples and the 0.1 mg/L control from your best-fit line. Concentration of Lake Osceola, inlet sample = ___________ mg/L Concentration of Lake Osceola, outlet sample = ___________ mg/L Computed Concentration of 0.1 mg/L control = _____________ mg/L Determine the percent error for the control sample. (Show work below) DISCUSSION AND CONCLUSIONS Discuss data quality and potential sources of error....
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This note was uploaded on 01/08/2012 for the course CAE 345 taught by Professor Staff during the Spring '11 term at University of Miami.

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shphosphate_04 - On a separate sheet graph the standard...

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