Lab Report Example

Lab Report Example - and allowed to sit for two minutes...

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Name: Laboratory Report: In this experiment we aimed to determine which biological macromolecules (reducing sugars, starch, and proteins) are present in milk. Furthermore, we hypothesized that milk would test positive for reducing sugars, starch, and proteins based on the nutritional information for milk that was provided at the end of the laboratory manual. Procedure: 1. Labeled six test tubes RS+, RS-, S+, S-, P+, P- (Positive and Negative Controls for reducing sugar, starches and proteins respectively). 2. Added 2 mL of Glucose Solution to RS+, 2 mL of Starch Solution to S+, 2 mL of Egg Albumin solution to P+. 3. Added 2 mL of diH 2 O to RS-, S-, and P-. 4. Labeled three test tubes 1, 2, and 3, and added 2 mL of milk to each. 5. Added 5 mL of Benedict’s Reagent to RS+, RS-, and tube #1, and boiled the tubes for three minutes. 6. Added one dropper full of Iodine to S+, S-, and tube #2, and agitated the tubes. 7. Added 2 mL of Biuret’s Reagent to P+, P-, and tube #3, gently agitated the tubes
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Unformatted text preview: and allowed to sit for two minutes. Results: Macromolecules Present in Milk Test For Coloration of Positive Control Coloration of Negative Control Milk (+ or -) Red. Sugars Reddish Brown Blue + Starch Bluish Black Brownish-Proteins Purple Blue + Figure 1. Using detection reagents to determine the presence or absence of macromolecules in milk. Milk tested positive for both reducing sugars and proteins, but it tested negative for the presence of starch. Conclusion(s): Our hypothesis stating that reducing sugars and proteins are present in milk was supported by the positive result observed. However, our hypothesis stating the starch would also be present in milk was invalidated by our results. The obtained result could be due to either the absence of the macromolecule in milk, or an error committed while performing the steps of the protocol. Repetitions of this experiment could further elucidate the validity of both the positive and negative results obtained....
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This note was uploaded on 01/06/2012 for the course BIOL 1406 taught by Professor Markgarcia during the Fall '11 term at Collins.

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