Spectrophotometry Worksheet A

Spectrophotometry Worksheet A -...

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Unformatted text preview: Spectrophotometry
Worksheet
 
 
1.

Below
are
listed
either
ranges
of
light
or
colors
of
light.

In
the
instance
of
a
 range,
state
what
color
of
the
visible
spectrum
that
you
would
expect
to
see.

In
the
 instance
of
a
color,
please
state
what
range
of
wavelengths
transmits
that
particular
 color.
 
 Green
 
 Red
 


 380‐430
nm
 
 590‐620
nm
 
 2.

Please
define
the
terms
“transmittance”
and
“absorbance.”

 
 
 
 
 
 
 
 
 
 3.

When
you
needed
to
set
the
spectrophotometer
to
0%
transmittance
initially,
 what,
if
any,
sample
did
you
have
inside
of
it?

When
you
needed
to
set
the
 spectrophotometer
to
100%
transmittance,
what
if
any,
sample
did
you
have
inside
 of
it?
 
 
 
 
 
 
 
 4.

Please
define
the
term
“serial
dilution.”

Why
did
we
use
serial
dilutions
in
the
 spectrophotometry
lab?
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 5.

You
start
with
a
solution
that
is
60
mg/ml
of
protein
and
perform
a
series
of
 serial
dilutions
on
it.

Please
fill
in
the
table
below
with
the
appropriate
 concentrations
at
each
dilution.
 
 Concentration
(mg/ml)
 Absorbance
 1
 60
 .750
 2
 
 .340
 3
 
 .180
 4
 
 .08
 5
 
 .02
 
 6.

You
have
a
mystery
solution
that
has
an
absorbance
of
.250.

Using
just
the
table
 in
question
5
alone,
give
an
approximation
or
a
range
of
concentrations
that
could
 be
that
or
those
of
the
mystery
solution.

Please
justify
your
answer.
 
 
 
 
 
 
 
 
 7.

Now
graph
your
results
from
question
5
on
the
graph
provided
on
the
next
page,
 draw
a
best
fit
line,
and
then
determine
an
approximate
value
for
the
concentration
 of
the
mystery
sample.
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 ...
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This note was uploaded on 01/06/2012 for the course BIOL 1406 taught by Professor Markgarcia during the Fall '11 term at Collins.

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