Lab reports

Lab reports - REPORT #1 Spectrophotometry Name_ Section: M...

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REPORT #1 Spectrophotometry Name_______________________ Section: M T W R F 8 11 2 Relationship between wavelength and color : Write down the wavelength of each color. red___________ orange___________ yellow____________ green_________ blue_____________ violet_____________ % Transmittance and absorbance at (540 nm) for spectrophotometer calibration : % transmittance_________________ absorbance_________________ Development of a Standard Curve Before drawing your graph be sure you determine which, absorbance or transmittance, are you to use, and write on the y-axis the units. Table of absorbance/transmittance readings according to concentrations 0.625 mg/ml 1.25 mg/ml 2.5 mg/ml 5 mg/ml 10 mg/ml unknown Graph protein mg/ml (x-axis) vs. absorbance or transmittance (y-axis) Determine protein concentration (mg/ml) of the unknown. ____________ mg/ml. Did your result conside with the curve? Explain using back of page.
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REPORT #2 Acids, Bases and pH Name___________________________ Section: M T W R F 8 11 2 Making a set of Standards : Determine the colors of each pH. pH Colors 2 4 6 7 8 10 12 Determine the pH of unknown solutions comparing the colors to those of the standards. A___________ B____________ C___________ Measuring pH using pH meter : Determine the pH of each solution. Which of these are acids, basis or neutral solutions? Type of Solutions pH Which of these solutions was the most acidic? ____________________________ Which of these solutions was the most basic? _____________________________
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Determining the Buffering Capacity of a solution : State your hypothesis about which of these solutions will be a better buffer. _____________________________________________________________________________ _ Draw the graphs for both solution A and B using different colors and labeling them . 0 ml HCL added ml of NaOH added Which of the two buffers tested, A or B, has the highest buffering capacity?________________ State your conclusion regarding this last experiment ___________________________________ _____________________________________________________________________________
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REPORT #3 Macromolecules Name_____________________________ M T W R F 8 11 2 Chose 3 of the 6 solutions seen in class and fill in the information asked for below. Solution 1:______________
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This note was uploaded on 01/06/2012 for the course BIOL 1408 taught by Professor Markgarcia during the Fall '11 term at Collins.

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Lab reports - REPORT #1 Spectrophotometry Name_ Section: M...

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