{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

2- SPECTROPHOTOMETRY H.WS1L

# 2- SPECTROPHOTOMETRY H.WS1L - variable on y-axis Take data...

This preview shows pages 1–2. Sign up to view the full content.

SMURALI Biology 1408 Spectrophotometry Work sheet Name: Section: Label and describe the function (2pts) 1. Sample holder 2. 0%T Knob 3. 100% T knob 4. Wavelength control Knob Relationship between wavelength and color(2pts) What is the wavelength range for the given colors: Color red green blue yellow Wavelength Development of a standard curve-Draw the standard curve and plot unknown from your lab work Plot protein concentration in mg/ml (independent variable) on x-axis and absorbance (dependent

This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: variable) on y-axis. Take data from above table. • Plot unknown with different color pencil and show extrapolation to determine protein concentration. • Draw best fit line . Absorbance (A) 0.5 0.4 0.3 0.2 0.1 0.0 SMURALI Biology 1408 What is the purpose of blank reading and to what transmittance do you set it? Protein concentration (mg/ml) 0 1 2 3 4 5 6 7 8 9 10...
View Full Document

{[ snackBarMessage ]}

### What students are saying

• As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

Kiran Temple University Fox School of Business ‘17, Course Hero Intern

• I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

Dana University of Pennsylvania ‘17, Course Hero Intern

• The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

Jill Tulane University ‘16, Course Hero Intern