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2- SPECTROPHOTOMETRY H.WS1L

2- SPECTROPHOTOMETRY H.WS1L - variable on y-axis Take data...

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SMURALI Biology 1408 Spectrophotometry Work sheet Name: Section: Label and describe the function (2pts) 1. Sample holder 2. 0%T Knob 3. 100% T knob 4. Wavelength control Knob Relationship between wavelength and color(2pts) What is the wavelength range for the given colors: Color red green blue yellow Wavelength Development of a standard curve-Draw the standard curve and plot unknown from your lab work Plot protein concentration in mg/ml (independent variable) on x-axis and absorbance (dependent
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Unformatted text preview: variable) on y-axis. Take data from above table. • Plot unknown with different color pencil and show extrapolation to determine protein concentration. • Draw best fit line . Absorbance (A) 0.5 0.4 0.3 0.2 0.1 0.0 SMURALI Biology 1408 What is the purpose of blank reading and to what transmittance do you set it? Protein concentration (mg/ml) 0 1 2 3 4 5 6 7 8 9 10...
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