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ENZYMES-Lactase

ENZYMES-Lactase - • place tubes in 40 C •?Which tube...

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ENZYMES Introduction ?enzymes are proteins ?enzymes are catalysts ?speed up a chemical reaction by lowering the activation energy required ?thousands of enzymes in cells ?catechol oxidase will be tested
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Factors that Affect Enzyme Activity Factors that Affect EnzymeActivity for catechol oxidase ?specificity ?ability to discriminate between similar structures ?temperature?optimally, 40 C ?pH?optimally, pH 7 ?cofactors?Cu ++ (metallic ions
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Enzyme Structure and Function Enzyme Structure andFunction ?Enzyme (catechol oxidase in potato extract)?active site?allosteric site ?substrate (catechol) binds to active
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Enzyme Structure and Function, cont substrate and enzyme (lactose) (lactase) enzyme-substrate complex Products (glucose)
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Specificity Procedure ?tube L (enzyme + Lactose)) ?tube M (enzyme + Maltose)
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Unformatted text preview: • ? place tubes in 40 C • ?Which tube shows more product formed Temperature • Procedure • ?place enzyme in appropriate temp. for 5 min • before testing • ?tube at 5 C tube at 25 C?tube at 40 C tube at 60 C?tube at 80 C and tube at 100 C • ??Which tube shows most product formed pH • ?Procedure • ?Place enzyme in appropriate buffer for 5 min • before testing, place 40 C • ?tube at pH 2 tube, at pH 4,?tube at pH 6 tube at pH 7,pH 8,?tube at pH 10 tube at pH 12 • ??Which tube shows most product formed? Cofactors • ?place tubes in 40 C • ?tube C (enzyme + water + milksubstrate) • ?tube E (enzyme + EDTA + milksubstrate) • ??Which tube shows product formed? • ?*PTU (phenylthiourea is poisonous), Dispose in labeled containers...
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ENZYMES-Lactase - • place tubes in 40 C •?Which tube...

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