Photosynthesis

Photosynthesis - • baseline Chromatography Rf values •...

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Photosynthesis
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Photosynthesis light reactions (thylakoid) 6CO 2 + 12 H 2 O gives C 6 H 12 O 6 + 6O 2 + 6H 2 O Calvin cycle or dark reactions (stroma) ?photoenergy is required (endergonic
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Using the Spectroscope Using the Spectroscope ?view white light w/ spectroscope,record colors seen ?view red, blue, and green filters w/ Spectroscope,record colors seen ?view spinach extract w/ spectroscope,record colors seen
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Using the Spectrophotometer % tube 1 spinach extract tube 2 (blank) solvent tube 3 carrot extract ? record %T from 400-700 nm in 25nm increments, graph ? 100% T must be reset whenever λ is changed
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Chromatography solvent line carotenes = orange carotenoids xanthophylls = yellow chlorophyll a = blue-green chlorophylls chlorophyll b = yellow-green
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Unformatted text preview: • baseline Chromatography, Rf values • Chromatography, Rf values • ?Rf = pigment distance/solvent distance • ?affinity for the solvent = high Rf value • ?affinity for the paper = low Rf value • ?Staple finished chromatograms to report Starch localization in leaves • Starch localization in leaves • ?Boiling the leaf in alcohol will remove the • pigments, it will turn white • ?flood leaf w/ iodine • iodine + starch = blue-black • Draw leaf in color before boiling and after staining. Chloroplast Model • Label chloroplast in your lab manual • ?outer membrane • ?inner membrane • ?intermembrane space • ?stroma (Calvin cycle or dark reactions) • ?thylakoid (light reactions), granum, grana...
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This note was uploaded on 01/06/2012 for the course BIOL 1406 taught by Professor Markgarcia during the Fall '11 term at Collins.

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Photosynthesis - • baseline Chromatography Rf values •...

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