Photosynthesis

Photosynthesis - baseline Chromatography, Rf values...

Info iconThis preview shows pages 1–8. Sign up to view the full content.

View Full Document Right Arrow Icon
Photosynthesis
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Photosynthesis light reactions (thylakoid) 6CO 2 + 12 H 2 O gives C 6 H 12 O 6 + 6O 2 + 6H 2 O Calvin cycle or dark reactions (stroma) ?photoenergy is required (endergonic
Background image of page 2
Using the Spectroscope Using the Spectroscope ?view white light w/ spectroscope,record colors seen ?view red, blue, and green filters w/ Spectroscope,record colors seen ?view spinach extract w/ spectroscope,record colors seen
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Using the Spectrophotometer % tube 1 spinach extract tube 2 (blank) solvent tube 3 carrot extract ? record %T from 400-700 nm in 25nm increments, graph ? 100% T must be reset whenever λ is changed
Background image of page 4
Chromatography solvent line carotenes = orange carotenoids xanthophylls = yellow chlorophyll a = blue-green chlorophylls chlorophyll b = yellow-green
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 6
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 8
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: baseline Chromatography, Rf values Chromatography, Rf values ?Rf = pigment distance/solvent distance ?affinity for the solvent = high Rf value ?affinity for the paper = low Rf value ?Staple finished chromatograms to report Starch localization in leaves Starch localization in leaves ?Boiling the leaf in alcohol will remove the pigments, it will turn white ?flood leaf w/ iodine iodine + starch = blue-black Draw leaf in color before boiling and after staining. Chloroplast Model Label chloroplast in your lab manual ?outer membrane ?inner membrane ?intermembrane space ?stroma (Calvin cycle or dark reactions) ?thylakoid (light reactions), granum, grana...
View Full Document

Page1 / 8

Photosynthesis - baseline Chromatography, Rf values...

This preview shows document pages 1 - 8. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online