Prokaryotes

Prokaryotes - Staining ?grown in: broth and agar (Petri...

Info iconThis preview shows pages 1–11. Sign up to view the full content.

View Full Document Right Arrow Icon
Prokaryotes Prokaryotes ?Three domains ?Bacteria – prokaryotes ?Archaea – prokaryotes ?Eukarya – eukaryotes
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Two prokaryotic domains • Bacteria – typical heterotrophic bacteria, ? photosynthetic cyanobacteria ? pathogens Archaeobacteria? halophiles? hermophiles? acidophiles
Background image of page 2
Bacteria ?binary fission, type of reproduction peptidoglycan, cell wall composition flagella composed of flagellin,• ribosomes, small and free in cytosol nucleiod for a circular dsDNA ubiquitous colony – tens of thousands of organisms all originating from a single cell that underwent binary fission
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Bacterial Morphology ?Three basic shapes:• coccus,• bacillus • spirillum ? draw the three basic shapes
Background image of page 4
Coccus shape-spherical shape
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Bacillus-rod shape
Background image of page 6
Spirillum-spiral shape
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Bacterial Culture and Staining Bacterial Culture and
Background image of page 8
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 10
Background image of page 11
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Staining ?grown in: broth and agar (Petri plates or slants) ?Gram stain divides bacteria Gram positive- thick cell wall- retains crystal violet color Gram negative- double walls- retains counterstain safranin color Hans Christian Gram Staining Gram Collect bacteria with sterile inoculating loop ? Heat fix slide ? Stain 1. Crystal violet (primary stain) for 20 sec, rinse with water 2. Iodine (mordant) for 60 sec, rinse with water 3. Ethanol (decolorizer) dropwise, rinse with water 4. Safranin (counterstain) for 20 sec, rinse with water ? Blot dry with bibulous paper ? View at 1000x total magnification Biochemical Identification Compare your plates to the agar slants and petri dishes to identify the bacteria...
View Full Document

This note was uploaded on 01/06/2012 for the course BIOL 1406 taught by Professor Markgarcia during the Fall '11 term at Collins.

Page1 / 11

Prokaryotes - Staining ?grown in: broth and agar (Petri...

This preview shows document pages 1 - 11. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online