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Unformatted text preview: 5. Place 3ml filtrate into test tube + 3 ml 95% ethanol 6. Add phenol red and record color 7 DNA Electrophoresis a Restriction endonucleases or restriction enzymes (REs) ` Cut specific DNA sequences ` Produce blunt or sticky ends Escherichia coli RY13 Eco RI GAATTC sticky CTTAAG Haemophilus aegyptius Hae III GGCC blunt CCGG 8 Electrophoresis Applying voltage to solution of charged molecules for separation 1. Prepare gel, place in chamber 2. Introduce DNA sample a DNA is negatively charged, it moves to positive electrode a small fragments migrate faster than larger ones a accumulation of same size fragments show up as a band on the gel 3. Stain gel and record band pattern 4. Draw and label bands on report sheet...
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