biotechnology

biotechnology - 5. Place 3ml filtrate into test tube + 3 ml...

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1 Biotechnology a Biotechnology - process of manipulating the genome of an organism to form useful products a Genetic engineering – modification of genetic material done by genetic engineers a Sections ` Nucleic Acids ` Isolation of DNA ` DNA Electrophoresis 2 Nucleic Acids a Use puzzle pieces to show ` Replication (DNA to DNA): 5’ to 3’ ` Transcription (DNA to RNA): 5’ to 3’ ` Translation (mRNA to polypeptide): 5’ to 3’ 3 Replication: DNA to DNA DNA A T G C DNA T A C G 5’ 3’ 3’ 5’
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4 Transcription: DNA to RNA DNA A T G C RNA U A C G 5’ 3’ 3’ 5’ 5 Translation: mRNA to polypeptide tRNA A U G mRNA U A C Amino acid Tyrosine 5’ 3’ 3’ 5’ UAC = Tyr 6 Isolation of DNA From plant (onion) cells 1. 1.5 g salt + 90 ml water + 10ml detergent into 250 ml beaker 2. Chop onion, place in mixture 3. Place onion mixture in 60 ° C for 10 min and press 4. Filter onion mixture into clean beaker
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Unformatted text preview: 5. Place 3ml filtrate into test tube + 3 ml 95% ethanol 6. Add phenol red and record color 7 DNA Electrophoresis a Restriction endonucleases or restriction enzymes (REs) ` Cut specific DNA sequences ` Produce blunt or sticky ends Escherichia coli RY13 Eco RI GAATTC sticky CTTAAG Haemophilus aegyptius Hae III GGCC blunt CCGG 8 Electrophoresis Applying voltage to solution of charged molecules for separation 1. Prepare gel, place in chamber 2. Introduce DNA sample a DNA is negatively charged, it moves to positive electrode a small fragments migrate faster than larger ones a accumulation of same size fragments show up as a band on the gel 3. Stain gel and record band pattern 4. Draw and label bands on report sheet...
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biotechnology - 5. Place 3ml filtrate into test tube + 3 ml...

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