enzymes - ENZYMES Introduction enzymes are proteins enzymes...

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1 ENZYMES Introduction a enzymes are proteins a enzymes are catalysts ` speed up a chemical reaction by lowering the activation energy required a thousands of enzymes in cells ` catechol oxidase will be tested 2 Factors that Affect Enzyme Activity for catechol oxidase a specificity ` ability to discriminate between similar structures a temperature ` optimally, 40 C a pH ` optimally, pH 7 a cofactors ` Cu ++ (metallic ions) 3 Enzyme Structure and Function a Enzyme (catechol oxidase in potato extract) ` active site ` allosteric site a substrate (catechol) ` binds to active site
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4 Enzyme Structure and Function, cont. substrate + enzyme (catechol) (catechol oxidase) enzyme-substrate complex products enzyme (benzoquinone + water) + (catechol oxidase) brown color recovered 5 Prep of control and blanks Procedure a place tubes in 40 0 C ` tube A blank (enzyme + water) ` tube B blank (substrate + water) ` tube C control (substrate + enzyme) a Record results at 540 nm a keep tubes until the end of experiment 6 Specificity Procedure a place tubes in 40
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This note was uploaded on 01/06/2012 for the course BIOL 1406 taught by Professor Markgarcia during the Fall '11 term at Collins.

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enzymes - ENZYMES Introduction enzymes are proteins enzymes...

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