macromolecules

macromolecules - ` central C atom, amino group, carboxyl...

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1 Macromolecules Introduction a Four classes ` carbohydrates ` proteins ` lipids ` *nucleic acids *no t s tud ied 2 Carbohydrates a CHO a glycosidic linkage is between 2 sugars a groups ` monosaccharides ` disaccharides ` *oligosaccharides ` polysaccharides * not studied 3 Carbohydrates, cont. a Monosaccharides ` glucose, fructose, galactose a Disaccharides ` maltose (glucose-glucose) ` lactose (glucose-galactose) ` sucrose (glucose-fructose) a Polysaccharides enzymes
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4 Carbohydrates Reducing Sugars a Redox ` red = reduction (gain electrons) ` ox = oxidation (lose electrons) a reducing sugar - reducing agent, donates electrons to reduce a molecule ` all monosaccharides, some disaccharides 5 Benedict’s Test for Red. Sugars Cu ++ + red. Sugar Cu + + red. Sugar Benedicts red ox a Procedure: boil solution for 3 minutes (2ml substance + 2ml Benedict’s) ` blue ` green + ` yellow ++ ` orange +++ ` red/brown ++++ 6 Carbohydrates Iodine Test for Starch Procedure: a 1 ml Iodine + 2 ml substance a results ` blue-black + ` yellow-brown
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7 Proteins a CHON a monomer ` amino acid, 20 a amino acid structure
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Unformatted text preview: ` central C atom, amino group, carboxyl group, H atom, R group a peptide bond is between 2 a.a. 8 Biuret Test for Proteins a Procedure Mix 2ml substance + 2 ml Biuret a results ` light blue ` slight purple + ` deep violet ++ 9 Lipids a CHO a fat = glycerol + fatty acids (1-3) a water-insoluble (hydrophobic bonding) a nonpolar 10 Sudan IV Test Procedure a oil + water = emulsion a add Sudan IV, which layer does it stain? a add detergent (emulsifier), what happens? ` oil droplets are suspended in water 11 Hydrolysis of Macromolecules a hydrolyze: ` sucrose glucose + fructose ` starch maltose + maltose + … a fructose and maltose are red. sugars a hydrolysis will be tested with ` HCl ` HCl + heat 12 Hydrolysis of Macromolecules Procedure ` starch + HCl test for hydrolysis ` sucrose + HCl test for hydrolysis ` boil starch + HCl test for hydrolysis ` boil sucrose + HCl test for hydrolysis...
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This note was uploaded on 01/06/2012 for the course BIOL 1406 taught by Professor Markgarcia during the Fall '11 term at Collins.

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macromolecules - ` central C atom, amino group, carboxyl...

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