photosynthesis - Photosynthesis light reactions (thylakoid)...

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1 Photosynthesis light reactions (thylakoid) 6CO 2 + 12 H 2 OC 6 H 12 O 6 + 6O 2 + 6H 2 O Calvin cycle or dark reactions (stroma) a photoenergy is required (endergonic) 2 Using the Spectroscope a view white light w/ spectroscope ` record colors seen a view red, blue, and green filters w/ spectroscope ` record colors seen a view spinach extract w/ spectroscope ` record colors seen 3 Using the Spectrophotometer tube 1 spinach extract tube 2 (blank) solvent tube 3 carrot extract a record %T from 400-700 nm in 25nm increments, graph a 100% T must be reset whenever λ is changed
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4 Chromatography solvent line carotenes = orange carotenoids xanthophylls = yellow chlorophyll a = blue-green chlorophylls chlorophyll b = yellow-green baseline 5 Chromatography, Rf values a Rf = pigment distance solvent distance a affinity for the solvent = high Rf value a affinity for the paper = low Rf value a Staple finished chromatograms to report 6 Starch localization in leaves a Boiling the leaf in alcohol will remove the pigments, it will turn white a
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This note was uploaded on 01/06/2012 for the course BIOL 1406 taught by Professor Markgarcia during the Fall '11 term at Collins.

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photosynthesis - Photosynthesis light reactions (thylakoid)...

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