prokaryotes

prokaryotes - a Heat fix slide a Stain 1 Crystal...

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1 Prokaryotes a Three domains ` Bacteria – prokaryotes ` Archaea – prokaryotes ` Eukarya – eukaryotes 2 Two prokaryotic domains Bacteria – typical heterotrophic bacteria, ` photosynthetic cyanobacteria ` pathogens Archaeobacteria ` halophiles ` thermophiles ` acidophiles 3 Bacteria a binary fission, type of reproduction peptidoglycan, cell wall composition flagella composed of flagellin ribosomes, small and free in cytosol nucleiod for a circular dsDNA ubiquitous colony – tens of thousands of organisms all originating from a single cell that underwent binary fission
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4 Bacterial Morphology a Three basic shapes: coccus bacillus spirillum a draw the three basic shapes a examine the arrangement 5 Bacterial Culture and Staining a grown in: broth agar (Petri plates or slants) a Gram stain – divides bacteria Gram positive Gram negative 6 Hans Christian Gram Staining Gram a Collect bacteria with sterile inoculating loop
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Unformatted text preview: a Heat fix slide a Stain 1. Crystal violet (primary stain) for 20 sec, rinse with water 2. Iodine (mordant) for 60 sec, rinse with water 3. Ethanol (decolorizer) dropwise, rinse with water 4. Safranin (counterstain) for 20 sec, rinse with water a Blot dry with bibulous paper a View at 1000x total magnification 7 Hans Christian Gram Staining Gram a Collect bacteria with sterile inoculating loop a Heat fix slide a Stain 1. Crystal violet (primary stain) for 60 sec, rinse with water 2. Iodine (mordant) for 60 sec, rinse with water 3. Ethanol (decolorizer) dropwise, rinse with water 4. Safranin (counterstain) for 1-2 min, rinse with water a Blot dry with bibulous paper a View at 1000x total magnification 8 Biochemical Identification a biochemical tests – for definitive identification a Enterotube Multitest System, with • 12 media • 15 reactions...
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This note was uploaded on 01/06/2012 for the course BIOL 1406 taught by Professor Markgarcia during the Fall '11 term at Collins.

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prokaryotes - a Heat fix slide a Stain 1 Crystal...

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