Mitosis and Meiosis Lab - MITOSIS AND MEIOSIS Jennie Chang...

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MITOSIS AND MEIOSIS Jennie Chang Period 6 / / 1 11 12
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: PURPOSE The purpose of this lab is to study plant mitosis and calculate the . relative duration of the phases of mitosis in the meristem of root tissue , , Also to simulate the stages of meiosis by using chromosome models to study the crossing over and recombination that occurs during meiosis as well as the arrangements of ascospores in the asci from a cross between wild type Sordaria fimicola and mutants for tan spore coat color in this . fungus : MATERIALS microscope onion root tip slide whitefish blastula slide petri dish chromosome stimulation beads colored pencils : PROCEDURE . : Part 3A 1 1. . Examine the prepared slides of onion root tips or whitefish blastula 2. Locate the meristematic region of the onion or blastula with the . 10x objective 3. . Use the 40x objective to study individual cells 4. . Identify one cell that clearly represents each stage Draw and label . the cells . : Part 3A 2 1. Observe every cell in one high power field of view and determine . which phase of the cell cycle it is in Count at least two full fields ,. , of view If you have no counted at least 200 cells then count a . third field of view 2. . . Record your results in Table 3 1
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3. , Calculate the percentage of cells in each phase then record your . . results in Table 3 1 4. Calculate the amount of time the sell spends in each stage and . . record your results in Table 3 1 . : Part 3B 1 1. Study the process of meiosis by using chromosome simulation kits 2. : Interphase Place one strand of each color near the center of your work . area Bring DNA magnetic centromere region of one strand in contact . with the centromere region of the other of the same color Do the same . with its homolog 3. : . Prophase I Stimulate synapsis and the process of crossing over Pop the beads apart on one chromatid at the 5 th , bead or gene and do the . same with the other chromatid Reconnect the beads to those of the . . other color The visual result of crossing over is called a chiasma
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This note was uploaded on 01/09/2012 for the course BIO 87 taught by Professor Bio during the Spring '10 term at Aachen University of Applied Sciences.

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Mitosis and Meiosis Lab - MITOSIS AND MEIOSIS Jennie Chang...

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