Photosynthesis Lab - Photosynthesis Lab Jennie Chang AP...

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Unformatted text preview: Photosynthesis Lab Jennie Chang AP Biology Period 6 / / 11 18 11 : Background Info . Plants gain what they need through a process called photosynthesis . Photosynthesis uses energy from light captured by photosynthetic pigments , Water molecules are split in the process breaking apart for electrons and the . release of oxygen into the atmosphere as a by product The plants fix carbon from carbon dioxide into glucose and fructose chains and then the sugars can . combine to form long chains known as starches Many plants store their . photosynthetic products this way Some examples of photosynthetic pigments , , . include chlorophyll a chlorophyll b and carotene Most plants are approximately- . three quarters chlorophyll a and the rest is chlorophyll b The presences of the other pigments become noticeable when chlorophyll a and b no longer mask . ; their presence Chlorophyll a absorbs blue and red light chlorophyll b absorbs ; . blue green and orange red light carotenoids absorb blue and blue green light : Plants can lose water in two ways from inside the leaves through transpiration . and from the surface of the leaves or from the soil through evaporation To , , reduce water lost through transpiration leaves can close their pores or , . , stomata using special cells called guard cells However this also limits the , exchange of air and other gases which enter and exit leaves through the , . stomata so photosynthesis might also be limited Plants can adapt to this by , capturing sunlight s energy during the day and storing the energy until night and exchanging gases at that time when water loss is lower due to the lower . temperatures The first step in changing light to chemical energy is the . absorption of light by a pigment system Chlorophyll has the ability to absorb . light energy and produce an excited molecule The excited molecule of , chlorophyll b transfers its energy to a molecule of chlorophyll a which then . transforms it into chemical energy To measure the percentage of each , . wavelength of light absorbed by a pigment a spectrophotometer is used It directs a beam of light of a specific wavelength at the object to be analyzed and records what percentage of the light of each wavelength is absorbed by . the pigment or pigment system A spectrophotometer can also be employed to . measure the rate of photosynthesis Chromatography is a technique for , , analyzing or separating mixtures of gases liquids or dissolved substances such . , , , - as chlorophyll pigments For example column paper and thin layer . chromatography can be used to separate extracted plant and algal pigments In , paper chromatography the separation takes place through absorption and . capillary action The components of the spotted mixture move upward at , differing rates determined by both the solubility of the pigments in the solvent , and their relative attractions to the cellulose of the chromatography paper resulting in the different pigments in the mixture showing up as colored streaks...
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Photosynthesis Lab - Photosynthesis Lab Jennie Chang AP...

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