Chemistry 30A3 Fall 2011 Labs Dr. Troendle 42

Chemistry 30A3 Fall 2011 Labs Dr. Troendle 42 - 3....

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Chemical Biology 3OA3, September 2009 Page 42 3. E XPERIMENTAL P ROCEDURE : a. Washing and swelling the resin: Work as a pair for the first few steps. You have been given a tube containing 100 mg of the Rink resin on top of a porous frit. The frit allows solvent to pass through, while retaining the resin beads. Beneath the frit is a tap, and an exit tube. The resin is quite expensive: do not attempt to open the tube. Attach the exit tube to the rubber bung provided (CAUTION: use the correct procedure to insert the glass tube into the bung). Place the bung into a 250 mL Buchner flask, and attach the side arm to the laboratory vacuum supply. Your TA will demonstrate how to set up this apparatus initially. Close the tap, and turn on the vacuum SLIGHTLY to provide a weak suction. 1. Wash the resin with 3 separate aliquots of 1.5 mL of dichloromethane (DCM). Throughout this lab, the term “Wash” means add the solution to the resin, swirl gently
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