Chemical Biology 3OA3, September 2009 Page 43 the vial. Add a solution of di-isopropylethylamine (DIPEA, 8 equivalents) in DMF (0.5 mL). Stir thoroughly to dissolve the ingredients. 9. Add this solution to the resin in the column. Swirl as above with the rod. Allow this reaction to take place for 15 minutes. Swirl occasionally to mix the reagent with the resin. 10. Remove the liquid by aspiration, as above. 11. Wash the resin with 4 separate aliquots of 1.5 mL of dimethylformamide (DMF). OPTIONAL : it is possible to check for complete coupling at this point by transferring a few beads of resin into a test tube containing approx. 1 mL of 0.5% ninhydrin in ethanol solution. Heat for 10 min at 75-80 degrees. A blue colour indicates free amino groups on the resin, implying incomplete coupling. d. Splitting the resin: 12. Add 1.5 mL of dimethylformamide (DMF) to the resin. Stir, and use a Pasteur pipette to transfer half of the beads into a new apparatus. Use more DMF if needed.
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