Chapter 20

Chapter 20 - Chapter 20: Biotechnology 20.1: DNA cloning...

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20.1: DNA cloning yields multiple copies of gene or other DNA segment - DNA cloning and its applications: A preview o Step 1: isolate the plasmid of bacteria o Step 2: put DNA from another organism Now it’s called a recombinant DNA because it has 2 DNA o Step 3: plasmid is put back into the bacteria Producing recombinant bacterium> producing clones of cells with the recombinant plasmid o Gene cloning= production of mult. copies of a single gene Advantage: useful for… 1) making copies of a particular gene 2)producing a protein product - Using restriction enzymes to make recombinant DNA o We need an enzyme that cuts the DNA so we use restriction enzymes= protect the bacterial cell by cutting up foreign DNA from other organism or phages very specific because it recognizes restriction sites=particular DNA sequences o restriction fragments=a set of this is yielded when a restriction enzyme makes many cuts in the DNA molecule the restriction enzyme can be used over and over again on the gene you want o the most useful restiction enzyme cleave the sugar-phosphate backbones in 2 DNA strands in a staggered manner - Cloning a euk. gene in a bacterial plasmid
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Chapter 20 - Chapter 20: Biotechnology 20.1: DNA cloning...

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