Enzyme_lab_report

Enzyme_lab_report - EFFECTS OF PH AND TEMPERATURE ON ENZYME...

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EFFECTS OF PH AND TEMPERATURE ON ENZYME ACTION AND PRODUCT GENERATION ABSTRACT The action and production of the enzyme, alkaline phosphate, on the substrate, para-nitrophenyl phosphate or pNPP, was assayed on the pH levels ranging from 4.0 to 12.0, and at temperatures ranging from 0°C to 100°C. The enzyme had an optimum pH of 10.0 and an optimum temperature of 37°C. These findings proposed that lactic acidosis would interfere with the normal action and production of alkaline phosphate. INTRODUCTION Enzymes generally work best under certain narrowly defined conditions, such as appropriate temperature, pH, and ion concentration. Any departure from optimal conditions adversely affects enzyme activity. These factors affect the molecular conformation of the protein, as the hydrogen bonds responsible for its secondary, tertiary, and quaternary structures are broken. Enzymatic reactions occur slowly or not at all at low temperatures and the reaction rate increases as temperatures increases, yet some high temperatures may denature the reaction. Also most enzymes are only active around the optimal pH of the enzyme (Solomon, Berg, Martin, 2010). MATERIALS AND METHODS
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This note was uploaded on 01/09/2012 for the course PHYSICS 326 taught by Professor Staff during the Fall '08 term at Rutgers.

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Enzyme_lab_report - EFFECTS OF PH AND TEMPERATURE ON ENZYME...

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