Exam 3 - Exam 3 Introduction to Microbiology MICR 2123...

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Unformatted text preview: Exam 3. Introduction to Microbiology MICR 2123 November 2, 2011 Name: ' 7 7‘“ Part |. Multiple Choice. Circle the letter of the one most correct answer for each. Worth 2.5 points each." 1) The laws of thermodynamics tell us that systems tend to become less ordered and that which of the following in the universe always increases? a. Enthalpy (H) ® Entropy (S) c. Gibbs free energy change (AG) d. Energy of activation (Eact) 2) The largest amount of ATP made by substrate-level phosphoglation is produced during one round of which of the following? @Embdon-Meyerhoff—Parnas Pathway b. Entner—Doudoroff Pathway c. TCA cycle (:1. FoFl ATP Synthase 3) Just because a reaction can occur spontaneously doesn’t mean it will occur rapidly. To ensure that reactions occur within a reasonable biological timescale, cells use enzymes which are catalysts that lower the: a. Gibbs free energy change (AG) b. Entropy (S) c. Reduction potential (E°) @Energy of activation 4) The E yield from the Emben-Me erhoff-Parnas EMP athwa is: @ 2ATP+2 NADH b. 1ATP+2 NADH C. 1 ATP + 1 NADH + 1 NADPH d. 2 ATP + 1 NADH + 1 NADPH 5) Which of the followin would be most liker to start the electron transport system for a lithotroph? a. NADH H25 c. Pyruvate d. Nitrate 6) Which of the following could be used to m the electron transport system during anaerobic respiration? a. Oxygen b. NADH @Sulfate d. Ammonia 7) Which of the following does n_ot occur during the TCA cycle? {5‘ J’NADH is generated b. C02 is released c. ATP is made (‘yNADPH is made 8) Which is a measure of the tendency for an oxidized compound to accept electrons? a. Change in Gibbs free energy (AG°) “@‘Chang in entropy (AS) Reduction (Redox) potential (E°) (:1. Energy of activation (Eact) 9) The most rapid form of evolutionary change within microorganisms occurs by which of the following processes? I a) Mutation b) Vertical gene transfer (@Horizontal gene transfer d) Reductive genetics 10) Which of the following is used by prokaryotic Cyanobacteria for fixing C02? Calvin cycle b. Embden—Meyerhof—Parnas pathway c. Nitrogenase complex d. Entner-Doudoroff pathway 11) Which of the following provides an environment for the fixation of carbon in Cyanobacteria? ACarboxysomes b. Nitrogenase complex fiThylakoid membranes d. Inclusion bodies iwtggp‘n arm of the nitrogen triangle? ’“iih'fimu‘ c. Nitrogen fixation d. Denitrification 13) A specific Actinomycete that has a wax cell wall does not form endos ores and is responsible for w causing human disease on a massive scale is: is; ‘ . . ’6’; . . (9;. Mycobacterlum tube/’CU/OSIS (Samone/la typhlmurlum c. Bacillus anthracis i d. Clostridium botulinum 14) An organism growing on glucose obtains the bulk of its energy from substrate—level phosphorylation and releases propionic acid as a final product. Which metabolic process is occurring? a) Anaerobic respiration @Fermentation c) Aerobic respiration d) Lithotrophy 15) The key enzyme complex for the fixation of nitrogen (N2) is: a) ATP synthase b) RuBisCO c) Cytochrome c Q) Nitrogenase 16) Which of the following biochemical processes is an early step in cheese making and acts by causing the proteins in milk to coagulate (form solids)? a) Proprionic acid fermentation b) Anaerobic respiration c) Pasteurization @Rennet proteolysis 17) Which of the following groups species contains microganisms that cause important diseases such as a nthrax? Firmicutes b) Proteobacteria c) Pseudomonas d) Actinomycetes 18) Which of the following provides a concrete record of ancient forms of microorganisms? a) Phylogeny b) Horizontal gene transfer @Stromatolites d) Natural selection 19) Which of the following ism an important part of photolysis? m . /" “a 3) Reaction center /@ Electron transport system c) Antenna complex (9) Carboxysome 20) Which of the following is an organism that belongs to the Enterobacteriaceae group within the Gammaproteobacteria? @Escherichia coli b. Mycobacterium tuberculosis c. Streptococcus pyogenes d. Clostridium botulinum Exam 3. Introduction to Microbiology MICR 2123 November 2, 2011 Name: Part II. Fill in the blank. Complete the following lists or fill in the blank sentences with the most correct answer. Points are indicated for each and partial credit is possible. would be the term used to describe the general process of using <3 cellular energy to synthesize new molecules. (3) “"17 would indicate that a given chemical ’\ ‘ c v reaction should be able to occur spontaneously, though not necessarily quickly. (3) 23) M {V are the membranes within Cyanobacteria that contain the complexes ’1‘ ll! needed to generate chemica energy” from light. (3) '7 . , (group of bacteria) contain many members that are "a capable of forming endospores. (3) 25) More than half of the antibiotics currently used in medicine come from the group of bacteria V known as the QJN 26) The stress put upon a water source (such as a lake) caused by the introduction of excess nutrients (for example, fertilizer run—off or organic pollution) is called the CJVa/‘Zfit-wfl‘“ x . (3) 27) Name two things that the formation of organic acids during fermentation of foods contributes to the final food product: (4) a) bani-2:7 " b) “7.151% 7' 28) In most bacteria, the energy-containing electron carrier 7).} is used for biosynthetic reactions while the energy-containing electron carrier V ‘ if" feeds electrons and protons into the electron transport chain. (4) 29) What organism was mentioned in class as being responsible for the most recent large scale food- ...6 berm the Unitedagates? (2) ’1 r ; fl,“ y. ’ ,\ cmt iif z t ‘ 5 I, ,.i. ,m ,1 'i Lag, g Q\ i: 1?, I, VIA/bu“ 30) What is the type of fermentation that occurs during the production of wine or beer? (3) 7 D 32) Briefly [email protected] protein motive force is generated. Limit your answer to the space provided. 33) What membrane-associated enzyme complex is used by the proton motive force to make chemical energy inside a bacterial cell? (4) M‘ 34) Name the environment mentioned in class where you would expect to see the Entner—Doudoroff (ED) pathway in action. (3) ,/"’” ...
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Exam 3 - Exam 3 Introduction to Microbiology MICR 2123...

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