Final Exam - $353 APRIL 28 & 29 2011 FINAL EXAM-B...

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Unformatted text preview: $353 APRIL 28 & 29 2011 FINAL EXAM-B SECTION N0" MICRO 2132 FALL 2010 Name l. Motility medium Is light sensitive Contains an indicator to better visualize bacterial movement Is inoculated with a loop A and C a. b. C. d, r A and B 2. The ability to detect coagulase is important due to its effect on the virulence of certain bacteria, {l True False 3. Which of the following is a form of the Coagulase test a. Tube test b. Slide test A. Broth test A and B e. B and C 4. Blood agar plates can be very useful for the identification of Streptococcus Species. 5, Hemolysis is due to , 1 The production of exotoxins called hemolysin bk 'he production of endotoxins called hydrolysates c ‘he production of exoenzyrnes called hemolypases d. ‘he production of exoensymes called coagulases e None of the above. 6. Hemolysis is the destruction of white blood cells and hemoglobin. a. True , False 7. Lysine iron agar can determine a. Decarboxylation of Lysine b. Deamination of Lysine c. Glucose fermentation cl. Sulfur reduction All of the above 8. Lysine Iron agar requires two different inoculation techniques. V True ‘b'.’ 17. 18, 19. 20. 21. 22‘ 23. 24. A positive test result for the DNase test will be seen as a b. e. Good visible growth only No growth and color Change A clearing in the medium around any growth A darkening of both the medium and the growth None of the above. The DNase test demonstrates an organisms ability to at b. d. e. Make Deoxyribonuclease Bind to Deoxyribonuclease Degrade Deoxyribonuclease Make Ribonuclease Degrade Ribonuclease To obtain accurate results from the Gelatin Hydrolysis test, incubated tubes should be a. b. 6) d. e. Carefully examined for any color change Placed in a 30C incubator for a period of time prior to taking results Placed at 20C or less for a period of time prior to taking results Left out on the bench top until the next lab period Caretullly viewed with a uv light The Gelatin Hydrolysis test uses Nutrient Gelatin agar which a. b. C. @ e. Is difficult to inoculate properly Has to be checked every 24 hours Can only be incubated at 4C Melts at temperatures greater than 25C Melts at temperatures greater than 20C The medium used in the correct answer to we“; a bht pink color for a positive result. I. ‘ True False is inoculated with a heavy inoculum and will produce Urinary tract pathogenic bacteria may be distinguished from other enteric bacteria i" .m a slew With EMB agar With Urea Hydrolysis test With catalase test With Dnase test None of the above The enzymes that are responsible for starch and gelatin hydrolysis are a. b. V e . Intracellular enzymes Hydrophobic enzymes ExhaceHuarenzynies Oxidative enzymes None of the above Starch hydrolysis is made possible by the enzyme a. b. d e. Catalase Peroxidase Amylase Dnase Oxidase 32. Utilization media are highly defined formulations designed to differentiate organism based on their ability to grow when an essential nutrient such as carbon or nitrogen is strictly limited. '7 True False 33. Simmons Citrate agar is designed to determine an organism’s ability to utilize citrate as a sole carbon source. True False 34. Simmons Citrate agar results can be adversely affected if a heavy inoculation is used. ’ True b. False 3S. Nitrate reduction produces only one end product. a. True False 36. A positive nitrate test will show the following a. A gas bubble in the Durham tube prior to adding any reagents b. Red color change after the addition of the liquid reagents c. No color change after zinc is added All of the above e; None of the above 37. The Nitrate Reduction Test includes the following reagents a. Reductase A and B and copper b. Superoxide A and zinc . Nitrous A and zinc Nitrate A and B and zinc e Nitrate A and B and copper 38. Chromogenic reducing agents are chemicals that develop color as they become oxidized. True l5. False 39. The test for Cytochrome C does not require any reagents a. True False 40. Cytochrome C is detected by the Catalase test Thioglycollate test a. b. @ Oxidase test Decarboxylase test 8. Deaminase test 49. SO. 51. 52. 53. 54, .2 Use the follow tale to answer questions 49 & 50. Lab tech Jerry Jack Jones performed an O—F testl He set up the test with 2 tubes of O-F medium. Jerry Jack did this procedure while he was listening to some rocking Christmas music on his new used Walkman, picked up at the local pawn shop for two bucks (dollars not deeri). The tubes were then incubated for 48 hours. Jerry Jack removed the tubes from the incubator and proceeded to record results. He was a very disappointed to observe that he had quite obviously been a little too much into the drum solo he had been listening to while inocuiating the media as 3 separate stab lines appeared in one tube and 4 in the other tube. In addition he sadly faiied to cover one of the medium with sterile mineral oil. The motility results will still be accurate. Since both tubes turned yellow Jerry Jack is sate in recording the results as oxidative metabolism, a True False Oxidation-Fermentation (O—F) medium is designed to differentiate bacteria on the basis of fermentative or oxidative metabolism of carbohydrates. True “b; Faise Fiuid Thioglycoilate medium was designed to determine bacterial a, generation time oxygen tolerance c. motility fermentation e. none of the above The oxygen gradient in Fluid Thioglycollate medium is created by autoclaving and the diffusion of oxygen back into the medium bl specific gravity and the diffusion of oxygen back into the medium (2. the bacterial growth and waste products settling out d. incubation and not shaking the medium e patience and magic In Fluid Thioglycollate medium, the presence of growth toward the upper portion of the media and some growth traveling through to the bottom of medium, tells you that the organism is a strict anaerobe aerobic microaerophilic facultative anaerobe results are inconclusive a. b. c. e. i Xylose Lysine Desoxycholate agar (XLD) is designed to be used to Isolate Gram'positive bacilli Identify Staphylococcus aureus from Escherichia CO/i Isolate and identify ShigeI/a and Providenc/a Isolate and identify Gram—negative cocci Identity Easter/chia bun/717’ from Estereichia eggeae (beige?) 64. Which of the following media were designed to be seiective for Gram—positive bacteria? ,ax.‘ Mannitol Salt agar and Dnase agar .- Phenylethyl Alcohol agar and Columbia CNA Blood agar ‘c! MacConkey agar and Mannitol Salt agar d. XLD Agar and Hektoen Enteric Agar e. Bile Esculin agar and EMB agar 65. Selective and differential media are used primarily for a; A positive identification r A presumptive identification c. Identification of new bacteria only d. Identification of hard to treat bacteria e. None of the above Final Bonus Questions 1. This bacteria is a known sporeformer and used as a pesticide. a, Escherichia coli (EC) b. Yersinia pest/s (Yb) €_ Bacillus thurigiensis (Bt) a‘. Lady gagaii (Lg) 2. Serratia marcescens has a bright red pigment, also it: Was used by the US. Navy in bio-warfare tests Uses Calcium hydroxide as its sole carbon source c. Cannot utilize dihydrogen oxide d. Is a spore former 3. This bacteria has been linked to food poisoning, especially fried~rice a. Marlnobacter hydrocarbo'noclasticus b. Mycobacterium leprae Bacillus cereus Desu/fovibriofisheril 4. This bacteria has been linked to mastitis in cattle and is found on the human skin. 1; Staphylococcus aureus b. Bacillus megater/‘um cl Chromobacter thetapondensfs d. Can/'5 lupus S. This bacteria has caused food poisoning in raw cookie dough Escherichia coli Helicobacter pylori C. Jeotgall desulfuricans d. Jeanc/aude vandamme 56. S7. 58. S9. 60. 61. 62. 63. Eosin Methylene Blue agar contains two dyes both of which serve dual roles of inhibiting Gram+ bacteria and reacting with rapid lactose fermenters to change the color of their growth. True False Maconkey agar is used to differentiate bacteria based on “heir ability to ferment lactose heir ability to grow on the medium Their ability to tolerate lactose Their ability to stain Gram+ Their ability to change the color of the medium 030.516 MacConkey agar is made with 2 dyes. Which of the following is the correct dyes and usage of these dyes in this media? Neutral red is a pH indicator and Crystal violet is an inhibitor Neutral red is a pH indicator and Safranin is an inhibitor Phenol red is an inhibitor and Congo Red is a pH indicator Methyl green is an inhibitor and Crystal violets is a pH indicator None of the above {Dan A positive result on Bile Esculin agar is a. No precipitate and good growth indicating tolerance to the bile A dark precipitate in the medium, indicating hydrolysis of the esculin A dark precipitate in the medium, indicating hydrolysis of the bile d. No precipitate and little growth indicating a Gram+ organism e. Bile Esculin agar was not used in this lab Columbia Ci\A agar contains a. Sheep blood as the inhibitor . Casein as the inhibitor Antibiotics as the inhibitor Sodium chloride as the inhibitor e. Beef extract as the inhibitor Mannitol Salt agar and Eosin Methylene Blue agar (EMB) are media that are both selective and diffe ential. True b. False Should the medium turn yellow around any bacterial growth on a Mannitol Salt agar plate, this is an indication of ‘ a. Glucose fermentation b. Mannose fermentation . Sucrose fermentation Mannitol fermentation e: Lactose fermentation Which of the following is the i_n_hibitor in Mannitol Salt agar? a. Phenol red b Agar c. Peptone Sodium chloride is" None of the above 41. The age of bacterial cultures (how long the culture has been growing on a medium) is critical for the follow tests MSA, EMB and Phenol Red Broth at @ Oxidase, Catalase and Gram staining "c; MR—VP, Citrate and Nitrate d. e. Malonate, urea, and decarboxylase fermentation, hemolysis, deaminase 42. Catalase breaks down the correct answer to #23 into Hydrogen and a sodium ion Hydrogen peroxide and flavoprotein Water and oxygen Oxygen and hydrogen Superoxide and hydrogen a. b. . e. 43. Catalase is a. A hormone that breaks down sodium hydroxide @ An enzyme that breaks down hydrogen peroxide c‘. An enzyme the breaks superoxide dismutase d. A catalyst that does nothing a. A catalyst the turns bacteria white 44. MRaMP test is designed to detect Acetoin production and mixed acid fermentation b; Acetoin production and guanidine production c. Mixed acid fermentation and gas production d. Mixed acid fermentation and NADH e. None of the above 45. The Methyl Red and Voges—Proskauer (MR—VP) medium is an agar. True 46. After inoculation a PR8 tube with glucose shows positive for fermentation only. The media will show the following when compared with the un‘inoculated control a. Control is red and inoculated tube is red b. Control is yellow and inoculated tube is pink, c._ Control is red and inoculated tube is clear Control is red and inoculated tube is yellow e”; Not enough information to tell 47. PRB contains a small tube inside the larger tube. This tube is a. Simply a test tube and has no function Inverted, known as a Durham tube and used to trap gas a; Used to help determine color change d. Inverted and used to encourage bacterial growth e. Used to aid the dispersal of byproducts 48. Phenol Red broth (PR8) is designed primarily to determine Gas production only Fermentation of carbohydrates and gas production Growth only Which carbohydrates will prevent bacterial growth None of the above m mew 254 A positive result with the Phenylalanine agar will show Bacterial growth turns yellow with no reagents added Bacterial growth turns purple with Kovac’s reagent added Bacterial growth turns yellow with oxidase reagent added Bacterial growth turns green with ferric chloride reagent added Bacterial growth turns green with no reagents added me» w 26. The Phenylalanine agar is used to detect a. Pyruvic acid production 3 Phenylalanine deaminase enzyme production WC”. Phenylalanine decarboxylase enzyme production (3. Ferric citrate production e. None of the above 27. An enzyme that catalyzes the removal of an amino acid’s amine group is known as a Deaminase b. Dehydrogenase c, Aminase d., Oxidase e. Reductase / 28‘ An enzyme that catalyze the removal of an amino acid’s carboxyl group is known as a at Carbonase b. Catalase Decarboxylase d, Aminoase e, Dehydrogenase 29. TheDecarboxylase test utilizes sterile mineral oil in order to , Create an anaerobic environment in. Make it easier to see any color change (3. To keep the medium from drying out d. To prevent gas bubbles from escaping e. None of the above 30. The inhibition of enzyme activity when the inhibitor competes with the substrate for binding sites is known as a. Substrate inhibition Competitive inhibition Enzymatic inhibition d. Incomplete inhibition e. Binding site inhibition 31. is similar enough to succinate to replace it as a substrate in one of the many reactions of the Kreb’s cycle. Malonate C d e Manitol Gluconate Citrate None of the above 10‘ 11. 12. 13. 14. 15. 16, Which of the following tactOrs allow Triple Sugar Iron agar to provide multiple test results. a. The way the slants are made b. The different concentrations of sugars c. The inoculation methods , All of the above e. None of the above Triple Sugar Iron agar can provide the same type of information from it’s results as a, O—F medium b. MR~VP medium Phenol Red Broth “d. Mannitoi Salt agar e. Starch agar Triple Sugar Iron agar is used to determine carbohydrate fermentation and sulfur reduction True 1:). False Which of the following are combination media? a. Dnase agar, Maionate broth, SIM medium SIM medium, Triple Sugar Iron agar, MRVP broth MRVP broth, Simmons Citrate Agar, Starch agar Starch agar, Triple Sugar Iron agar, Coagulase medium Lysine Iron agar, Nitrate Broth, Urea agar {Deni Combination media is A single medium that is used to determine 2 or more test results A medium containing multiple indicators A medium that utilizes a combination of inoculation techniques A medium that can be inoculated with more than one bacteria A medium that can use a combination lock than Kovac’s reagent is added to SIM medium after incubation to check for The production of indole b". The production of lysine The production of insuiin The production of sulfur The production of tryptophan (no.9 What are the 3 tests that can be determined from SIM medium? a. Motility, catalase, citrate ' b. Indole, oxidase, fermentation Sulphur, indole motiiity dI Sulphur, indole, malonate 8. None of the above Biackening of SIM medium indicates Acid production Motility a. b. Sulfur reduction e. Sulfur oxidation Bad bacteria ...
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This note was uploaded on 01/10/2012 for the course MICR 2132 taught by Professor Staff during the Spring '11 term at Oklahoma State.

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Final Exam - $353 APRIL 28 & 29 2011 FINAL EXAM-B...

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