Lab Midterm - MICRO 2132 MIDTERM EXAM OCTOBER 11 &...

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Unformatted text preview: MICRO 2132 MIDTERM EXAM OCTOBER 11 & 12, 2011 FALL 2011 (60 points) NAT/ml SECTION No___““~_”_ FILL IN YOUR NAME AND SECTION NUMBER. READ AND FOLLOW THE INSTRUCTIONS BELOW. READ EACH QUESTION CAREFULLY AND COMPLETELY BEFORE SELECTING AN ANSWER. YOU MAY WRITE ON THE EXAM. ON THE ORANGE SCANTRON FILL OUT: 1—Your name, with last name first 2—Your section number After completion of the exam and bonus question return all pieces of paper to the front of the room. Place each paper in the correct stack as follows: All exams go together All scantrons go together All bonus questions go together You need only 35 correct answers out of the 40 questions, to achieve the full 60 points on the written part of the exam, BUT YOU MUST ANSWER ALL QUESTIONS hWNi-I- Best wishes to each of you! 1. Cells are divided into prokaryotes and eukaryotes based on Gram positive and Gram negative nature size and complexity of the cells habitat and method of reproduction Both A & C None of the above (DELQD‘QJ 2. Sterilization, disinfection and decontamination are categories of germicidal control systems. Each category is a different level of effectiveness. The following levels of control correspond with these 3 categories as they are listed above: highest (or third) level, moderate(or' second) level and lowest (or first) level. a. True b. False 3. Which of the following is NOT a category of colony morphology a. elevation b. texture c. arrangement d. shape e. margin 4. Quadrant streaking is m the only technique that can be used to obtain isolated colonies a. true b. false 12. The process of heat fixing a bacterial smear 13. 14. 15. l6. l7. compo-m makes the bacteria stick to the slide can be overdone and negatively affect the staining results is only for drying the water from the smear Both A & C Both A & B When performing an acid-fast stain the carbol fuchsin stain was inadvertently replaced by an acid fuchsin stain which is a water based stain solution and does not contain phenol. All other staining procedures followed the acid-fast staining procedure correctly. What is the predicted outcome of this staining process? a. b c. d. e All cells will be pink/red All cells will be unstained There will be a mixture of pink/red/ & blue All cells will be stained by the counterstain None of the above The substance in cell walls that allows a bacterium react positive for the acid—fast stain is a. b c. d. e Mycolic acid Hyoluronic acid Muratic acid Hydrochloric acid None of the above The use dilution test is a standard procedure used to a. b. c. d e measure the number of bacteria measure the effectiveness of antibiotics measure the effective of disinfectants differentiate between certain bacteria determine levels of contamination Viewing samples of living organisms from areas such as lakes or streams, is challenging mainly due to a. the movement of some organisms b. amount of preparation the wet mounts take c. the time it takes to differentiate eukaryotes and porcaryotes d the lack of contrast between the organisms and their surroundings e All of the above The term colony—forming unit is a more correct description of a colony’s origin because a. b. C. d. bacteria can come from any place no one knows where bacteria first originated some colonies form from individual cells and other colonies form from pairs, chains or clusters of bacterial cells colony—forming unit is what is used in the formula for calculating cell density None of the above 25. To sterilize an inoculation loop with a Bunsen burner, the loop should be passed a. through the very top portion of the flame b through the tip of the flames inner cone c. through the middle portion of the flame d. through the lower portion of the outer cone e none of the above 26. Of the many methods for sterilization the following is the most effective a. steam b dry heat c. uv radiation d. hot water & soap e hydrogen peroxide 27. The use of controls in experiments provides a useful reference for interpretation of result. was created for the inexperienced individual is never necessary for interpretations of results always has a positive and negative control none of the above meU-m 28. The total magnification of a specimen is 18OOX. Therefore a. the objective is 60X and the condenser is 30X b the objective is 100X and the ocular lens is 20X c. the objective is lOOX and the ocular lens is 18X d. the objective is lOOX and the condenser is 18X e none of the above 29. The following can be used as a differential stain Simple stain Gram stain, Acid—fast stain Endospore stain All of the above (DQDCJ'DJ 30. Some cells are made less vunerable to phagocytosis by a. The production of endospres b The production of exoenzymes c The production of extracellular capsules d. The production of antibiotics e None of the above 31. Cell walls with little or no lipid content and a thick layer of peptidoglycan are found in Gram negative cells acid-fast cells immature cells Gram positive cells cells containing endospores mapc'm 39. Negative or acidic stains a. Would stain the bacterial cell b Would stain the capsules c. Would stain the endospores d. Are used to determine if a bacterial species is acid-fast positive e Would stain the background only or would not stain the cells 40. The professor asked his student, Bill, what could be interpreted from the positive endospore stain Bill just completed. What could Bill have told (correctly) his professor if there were a lot of free endospres present on the slide? a. The bacterial culture is pretty old b. The bacteria seen are Gram-negative c. The bacteria have a capsule d. The bacteria potentially came from a harsh environment e. Both A and D Enjoy your 617'ef EreaEfrom James, fiat/e fun But 63 5a e. . . .See everyone next weefl! 53 film AWfimmvwimmmzéflmmna Em . :- L. 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Wm“ M...“ w_Dm:.._ mmzmm>n-,ucm_uomm - yzmémw. wrmmq mmm E3395 2.5529 .2mEcodozm 02 mam m ,‘Jmlm:g-,ggi;;iuiKiwlwi.‘:.i:i;f!,i‘Mth.sialz;;,:z,;ww.mz+;gi.i;:;;.!mW1mxili. >mOUm >mocm >woum >mocm >mnom d@G@®® :®®Q@@ 3®®@Q@ 3@®@0@ a®®®®® >moam bwncm >mocm >mncm >mOUm N§@@®@ aQ®®®Q m80®®® $@O@®@ $@@®@@ pmomm >wocm >w00m >woom Danum w®@3®@ ;@®@0@ a0®®®® $9®0®® a®®®®® >mOUm >moum >mnom >moom >wocm bO®©®® :Q®@®© §®O@@@ :®0@®@ g®@@®@ >mODm >w00m >mocm >wocm meUm m®®@®@ s®®¢®® m9.@®® aG®O®® a®®®®® >moom >woum >moom >wnom >moom m®®@fi@ a.®@®@ aQ®®®© a6®®0® g@®@®@ pmoum >woum >mOUm >m00m >m00m NO®®¢® :@®0@@ 20®®®® $O®@®@ 29®®®® >mocm >anm bmocm >wocm >wocm m®0@@@ a®®0®® g@®¢@@ aQ®®O® g®®®@@ >woom >moom >wOUm >woum >woum m®®@®0 s®®0®® g®®@®0 $®®O®@ s®®®®® >moom >mocm >wocm >woom >wOUm a®®®0® gG®©O® g®®0@@ s®®®®0 g®®@@@ >woom >mOUm >moum >m,OUm >mnum a®®®¢® a®®®®® :@®@®@ a®®®®® 2®®@®@ >mocm >woum >wo_um >mOUm >wocm $@@@®@ s®®®®® n®®@®@ s®®®®® sG®®®® >wocm >wOUm >woum >wOUm >woom a®®®®® aG®©®® :®®©®® a®®®®® a®®®®® >wOUm >wocm >moom >mOUm >moom g®®@®@ aQ®®®® :9®@®® i®®®®© :@®®@@ >wocm >mocm >woom >moom >anm aG®®®® aG®®®® a®®®®® a®®®®® a®®®®® >moom >mocm >moum >mOUm >woom aG®®®® a®®©®® :®®@@@ a9®©®® g®®@®@ >woom >m00m >moum >wOUm >mOUm a®®®®® a®®®®® :9®@®@ s®®®®® aQ®®®® >mocm >mocm >mocm >mOUm >WOUm a9®®®® aG®®®® a9®®®® g®®®®@ g®@@@@ meEm meUm >wo0m bwocm >WODm $®®@®@ g®®®®® a®®®®® s®®©®© g®®®®® >moom >mocm >moum >wOUm >WOUm a®®®®® a®®®®® s®®®®® g®®®®®ég®®®@@ _ _ m ~' w 2.”... fl H. mme w. 1 2132 CONNIE BUDD Page 13 Scan Number: 4 'X' indicates the question was omitted by the instructor. Student's Name: CULP JASON N n43 ID Number: 113—05— 026—1305026 1 2 “g é5_ > _ 4- , a .3, ' fi g, !. Score: 66.000 0707+“? 7h“ Percent Correct: 82.500 % Class Average 61.417 Class Median 60.000 Percentile Ranking 87.500 T—Score 56.795 QUESTION JMBER: 1 2 3 4 5 6 7 8 9 10 YOJR RESPO SE: B A C A D D A B E D CORRECT RESPONSE: B A C A D D A B E D QUESTION JMBER: ll 12 13 14 15 16 17 l8 19 20 YOJR RESPOWSE: C E D A C A C C C D CORRECT RESPO SE: C E D A C D C E A D QUESTION JMBER: 21 22 23 24 25 26 27 28 29 3O YOJR RESPO SE: D B A B B A A C E C CORRECT RESPO SE: C B A B B A A C E C QUESTION UMBER: 31 32 33 34 35 36 37 38 39 40 YOJR RESPO 8*: D B C B C D A D C E CORRECT RESPO SB: D B E B E D A D ~v * NAME ' STATION#1 (2pts) STATION#2 (2pts) STATION#3 (4DtS) STATION-#4 (2pts) STATION #5 (4PTS) MIDTERM FALL 2011 LAB PRACTICAL (40 POINTS) E1 SECTION 3‘” f1? WHAT IS THE GRAM REACTION AND CELL SHAPE SEEN ON THIS SLIDE? , “g, GRAM REACTION {3.1 T” CIJ’r”/7 SEW T“: TM, J CELL SHAPE WHAT IS THE STAINING TECHNIQUE DEMONSTRATED HERE? 23M Ci? 2 LA“ WHAT IS THE STRUCTURE (NOT THE BACTERIAL CELL——THE STRUCTURE) DEMONSTRATED IN THIS SLIDE? WHAT CELL ARRANGEMENTS ARE SEEN HERE? f 53 1 my V 7 f» “3 ' Q I r WHAT IS THE STAINING TECHNIQUE DEMONSTRATED HERE? {flu <1 “Vde WHAT IS THE PRIMARY STAIN SOLUTION USED IN THIS TECHNIQUE—THAT IS THE STAIN SOLUTION WHICH COLORS THE BACTERIAL CELLS FOR A POSITIVE RESULT pwa «WNW STATION#8 GRAM STAINING. YOU WILL DO THIS AT YOUR BENCH. YOUR SAMPLES ARE AT YOUR BENCH. YOU MAY DO AS MANY AS YOU HAVE TIME BUT MUST BE OUT OF THE LAB BY 5 MINUTE UNTIL THE HOUR. YOU ARE TO FIND THE SPECIMEN AND FOCUS ON IT BEFORE YOU CALL ONE OF US TO LOOK AT IT. SO CHOOSE CAREFULLY WHAT YOU WANT US TO LOOKAT. YOU ARE ALSO TO WRITE DOWN THE GRAM REACTION AND CELL SHAPE BEFORE CALLIN ON US TO LOOK AT THE SAMPLE (Spts-GRAM STAINING) . . ‘, :7. ‘ _" ‘ WM ; s. f \«"" SAMPLE# E1 GRAM REACTION . 1 ., v 5 CELL SHAPE (1AM T V 514‘ TA x": 5/“ STATION#9 QUADRANT STREAK PLATING. YOU ARE TO DO THIS AT YOUR BENCH. SAMPLE FOR THIS ARE AT THE BENCH AND LABEL “STREAK” AND THEY ARE RED. YOU MAY STREAK 2 PLATES IF NEEDED, BUT CAN INCUBATE ONLY l—YOU CHOOSE WHICH ONE TO INCUBATE. PLATES MUST BE INCUBATED CORRECTLY AND LABELED CORRECTLY. YOU WILL PUT THESE IN THE INCUBATOR YOURSELF. (5ptS-QUADRANT STREAKING) (Spts-ASEPTIC TECHNIQUE) TAB—M STREAK PATTERN ISOLATED COLONIES 15f}? 5L¢msampk 5Hufiom 14nlsanufle DHUfion: 1nfl 4L: i 9 m1 STATION#6 (SDtS) STATION#7 (6M5) BONUS: (2pts) SELECT TWO (2) PLATES AND GIVE THE COLONY MORPHOLOGY. A MINIMUM OF 4 CORRECT DESCRIPTIVES FOR FULL CREDIT. THERE ARE STERILE STICKS AND A COLONLY COUNTER WITH A BACKLIGHT AND MAGNIFIER FOR YOU TO USE IF YOU CHOOSE. PLATE# j: a». W ‘ w 69* x‘ . . , ‘f .. If,” . p‘ ( ‘ * r T k. “ , ,, f. . .. ,« v ‘2 " , : SGT I (“I R 2 f} I \4 5‘; :29}, /\ ml I IU iA /; PLATE# ; K r W H ’, i \ I/ v / a.” (M11941? x. (1:191; rnxfafi‘m“. J 9 Va / J (1)NAME THE PARTS NUMBERED AND (2)GIVE A MAJOR FUNCTION OF EACH. . £253 1'; ‘i “2 (gr; I m; ‘ ‘ w . p I.“ I _' ‘ 1,.\ "I .m . " ._ 3‘ ,, ‘ ' PART#l (1b 2 LIL 1?? fl, 3“»: 0g}: 31k Rafi _, : . n» . :1, ‘ :v 4/17 I ’ 1 \ i S 70". TIC; “ Nb. ‘w’ PART# 2 x n m ,, _ ' w . , J A. PART#3 Siwjg’htw 3v Hg. . _. Jr A? y,” IS THIS SPECIMEN A BACTERIA? FROM WHAT YOU CAN SEE AT THIS STATION BOTH IN THE SCOPE AND ON SCOPE, WHY P In ' ‘ I- 1 gm L , «I 3' .. , F. NOT? I; - \‘w‘ 5% 7 ‘l, 5 {an} {3?}; 3 {##233: ,.v .9 r Av, W “CE! I“ I“ :3“ ‘ “*1” ‘ I (ELLA N; ’1 ~ , V" I 32. Prior to heat fixing a bacterial smear, one should make sure a. the correct staining solution is used to make the smear b. the smear is air dried complete c. the smears are not over heated d. the bacteria are old enough to be heat fixed e. all of the above 33. The results of the Gram staining technique can be effected by a. The thickness of the bacterial smear b. The age of the culture c. The decolorization step d. The over- or under—heat fixing e. All of the above 34. What is the correct order of the phases in a normal growth curve? Log» Lag»Stationary~»Death Lag—’Log-’Stationary-#Death Stationary—rLagALog—rDeath Death+Stationary—>Lag—>Log Log ->Stationary—>Lag-*Death (Dario-m 35. The negative staining technique a. is used on bacteria to delicate to withstand heat fixing b. is used when an accurate size is crucial c. uses only 1 staining solution d uses the stain to make the bacterial smear e all of the above 36. When viewing an Endospore stained slide, are all pink stained bacteria Gram-negative? Yes, because Gram-negative bacteria stain pink Yes, because most spore forming bacteria are also Gram-negative No, because there should not be pink cells with this stain No, because both Gram—positive and Gram-negative cells would be stained pink. No because the cells will all stain green maps-m 37. For the purpose of Gram staining (and getting good results repeatedly) bacterial cultures should be 24 hours old or less. a. True b. False 38. The component of the Gram stain that acts as a mordent by binding with the primary stain IS 95% ethanol Safranin Crystal violet Gram’s Iodine None of the above mapo‘m You performed a serial dilution procedure. There was a series of 3 dilution tubes of (1:100) followed by 2 dilution tubes of (1:10). You then made a spread plate with 0.1ml of diluted culture from the final dilution tube. When the colonies were counted on the plate, there were 150 colonies. Use this problem to answer the following questions 33-35. You may draw/write as needed on the exam. 18. What is the dilution factor of the tube the spread plate was made from? a. 10'5 b 10'3 c. 10‘8 d. 10‘7 e 10‘9 19. What is the final dilution factor of the spread plate? a. 10‘10 b. 10'5 c. 10'9 d. 10'6 e. 10'7 20. What was the concentration of cells in the original sample or OCD? a. 1.5 x 108 b. 2.5 x 1010 c. 1.25 x 109 d. 1.5 x 1011 e. 1.25 x 106 21. Population density of bacteria can be estimated with the procedure of a. Quadrant streaking Culturing bacteria on an agar medium Standard plate count b c. d. All of the above e None of the above 22. A countable plate is one that contains between a. 10 and 200 colonies b. 30 and 300 colonies c. 25 and 500 colonies d 45 and 150 colonies e 20 and 300 colonies 23. The spread plate technique can be utilized for quantitative procedures a. true b. false 24. What is the correct formula for calculating OCD (original cell density)? a. CFU/ml = (dilution factor) X (diluent volume) b. CFU/ml = (Number of CFUs counted)/ (dilutions factor) X (volume plated) c. CFU/ml=(Number of CFUs counted) / (volume plated) d CFU/ml = (Number of CFUs counted) X (1/dilution factor) X (volume of original culture) e. None of the above 5. Which of the following in N_OT part of correct aseptic technique organization minimizing potential contamination hold open tubes at an angle adjust the Bunsen burner so it has only on ‘ congm ,WKW‘ ' was; when opening a plate use the lid as a shiefifim oi. ;. ‘ «»_l+.»«w maps-m 6. Colony morphology although greatly influenced by environmental factors it is determined by a. nutrient availability incubation time ratio of light and dark genetics percentage of oxygen available (napo- 7. Microorganisms that do not reside on or in a specific plant or animal host and are not known to cause disease are free—living are pathogenic are free-living are commensal are pathogenic are found only in a specific reservoir are free—living None of the above maps-m 8. Why do Basic stains bind to bacterial cells? The chromophore creates hydrolytic bonds with the Auxochrome Basic stains are positively charged and bacterial cell walls are negatively charged. Basic stains repel bacterial cells due to their net charge Basic stains are anionic and bacterial cells cationic. No one knows--—it's magic. mqpcrm 9. The antimicrobial susceptibility test is a standardized test for measuring the effectiveness of antimicrobics. It is a standardized test because Cultures are all the same genus, the plates are all the same thickness Of all the previous research performed using this method Uses liquid antimicrobics, the culture is diluted to a preset standard None of the above. The agar plates are all the same thickness, the antibiotic disks are standardized doses, and the bacteria inoculum is diluted to a preset standard met-acre: 10. The antimicrobial susceptibility test is also known as the a. Little disk method b Mcfarland method c. Mueller hinton method d. Kirby-Bauer method e Pain in the neck method 11. The Simple staining technique Determine genus and species of bacteria Effects the virulence factor of bacteria Uses only 1 stain solution 15 always a differential stain None of the above (papa-m ...
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Lab Midterm - MICRO 2132 MIDTERM EXAM OCTOBER 11 &amp;amp;...

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