13 - Cell Cycle

13 - Cell Cycle - The overall logic of the cell cycle: copy...

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Figure 17-1 Molecular Biology of the Cell (© Garland Science 2008) The overall logic of the cell cycle: copy DNA, segregate DNA, divide in two
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Figure 17-4 Molecular Biology of the Cell (© Garland Science 2008) The cell cycle is divided into phases
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Figure 17-14 Molecular Biology of the Cell (© Garland Science 2008) Cell cycle phases are separated by transitions that are driven by changes in cyclin-dependent kinase activity
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3 complementary approaches set the stage for our current understanding of cell cycle Hartwell et al Science 1974 183:46 Genetic Approach:
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3 complementary approaches set the stage for our current understanding of cell cycle Approach II: Discovery of Maturation-Promoting Factor (MPF) Harvest cytosol from here Inject here
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Approach III: Discovery of cyclins through evaluation of post-fertilization protein synthesis in sea urchin and surf clam embryos cell divisions ‘cyclin’
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25’ 95’ 25’ 105’ continuous labeling for: 10’ pulse ending at: Approach III: Discovery of cyclins through evaluation of post-fertilization protein synthesis in sea urchin and surf clam embryos
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MPF = Cdc2/Cdc28 Xenopus egg extract: deplete Cdc2 ( ) Cell. 1988 Jul 29;54(3):423-31. The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis. Dunphy WG, Brizuela L, Beach D, Newport J. Has MPF lacks MPF!
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Figure 17-15 Molecular Biology of the Cell (© Garland Science 2008) Cyclin-dependent kinases are protein kinases that are activated by a cyclin 1 in yeast 4 in human 6-10 in humans-yeast
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Cdk enzymes modulate the function of substrates by at least 3 general mechanisms P complex formation P complex disassembly P conformational change
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Science. 2009 Sep 25;325(5948):1682-6. Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution. Holt LJ, Tuch BB, Villén J, Johnson AD, Gygi SP, Morgan DO.
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This note was uploaded on 01/12/2012 for the course BI 9 taught by Professor Aravin during the Spring '10 term at Caltech.

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13 - Cell Cycle - The overall logic of the cell cycle: copy...

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