Rec II

Rec II - Bi9 Recitation 2 Prepared by Say-Tar Goh and...

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Bi9 Recitation 2 Prepared by Say-Tar Goh and Raymond Liu
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Recitation outline Paper for problem set 2 ( E. coli Pleiotropically Defective in the Export of Secreted Proteins by Oliver and Beckwith, 1981) Explain Methods Go through some figures A few more methods pertaining to Monday’s lectures
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A general workflow for mutagenesis and screening: Any organism Parent • Parental strain • All genes needed for survival under normal conditions Mutate • Generate mutations • Classic: random mutagenesis, can be site-specific Screen • Select phenotype • Classic: Infer genotype from phenotype • Modern: Sequence everything! (Can be limited)
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Parent • MC4100 • MM18 (Previously used, low B-Galactosidase) Mutate • Given enough cycles, E.coli generate mutations on their own Screen • Select phenotype: higher beta-gal activity (red) • No choice of “sequence everything” available, hence must infer from prior knowledge
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This note was uploaded on 01/12/2012 for the course BI 9 taught by Professor Aravin during the Spring '10 term at Caltech.

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Rec II - Bi9 Recitation 2 Prepared by Say-Tar Goh and...

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