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Lecture - Salmonella

Lecture - Salmonella - Food-borne Illness...

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Food-borne Illness
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http://www.youtube.com/watch? v=s7_QPeFYTx8&feature=fvsr
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Gram negative Rod shaped (bacillus) Non-spore forming Motile (many flagella) Enterobacteria (Live in the intestines of many animals) Chemoorganotrophs Salmonella infections are zoonotic
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http://en.wikipedia.org/wiki/Salmonella
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Laboratory staining protocol developed to help identify bacteria Two stains are used on heat-fixed (death by heat) smear of a bacterial culture Stain #1 is crystal violet which stains the bacterial cells purple Stain #2 is usually safrarin which stains the bacterial cells red or pink Gram + bacteria appear purple under microscope because they retain the crystal violet dye in their cell walls Gram- bacteria appear red or pink under the microscope because they do not retain the blue dye, but do retain the pink dye Does not work on all types of bacteria
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A Gram stain of mixed Staphylococcus aureus (Gram positive cocci) and Escherichia coli (Gram negative bacilli) http://en.wikipedia.org/wiki/Gram_staining
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