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Biol 112 Oct.28 (C)

Biol 112 Oct.28 (C) - (yeast ,studentedited Galactose...

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28/10/2011 1 Eukaryotic transcription regulation Saccharomyces cerevisiae (yeast) Image from MicrobeWiki, student edited microbiology resource 5 enzymes are required to metabolize galactose to glucose in yeast so that it can be used as an energy and carbon source Enzymes are only present when galactose is present and glucose is not present Galactose metabolism as a model system: GAL1 GAL7 GAL10 GAL1 GAL7 GAL10 DNA mRNA protein GAL1 GAL7 GAL10 GAL1, GAL7, GAL10 are unlinked and on different yeast chromosomes Logic of gene regulation – same in eukaryotes like yeast as in prokaryotes So the cell has genes with The cell also has RNA polymerases RNAPII GAL1 GAL7 GAL10 The GAL system is an example of how regulatory proteins control and coordinate gene expression in eukaryotes promoters and terminators Since everything is done by a proteins, the regulation is also done by proteins. Eukaryotic gene structure GAL1, GAL7, or GAL10 Basal transcription complex (RNAPII; TBP, other proteins) Regulatory protein binding Logic of coordinated gene regulation in eukaryotes GAL1 GAL7 GAL10 Devise a simple system for coordinate regulation of GAL1, GAL7, and GAL10 by a regulatory protein 1. What is the function of the regulatory protein? 2. How does the regulatory protein interact with GAL1 , GAL7 , GAL10 genes?
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28/10/2011 2 Model for coordinated regulation of eukaryotic genes GAL4 gene GAL4 protein (“transcription factor”) Inducing conditions (galactose present) GAL1 GAL7 GAL10 GAL1 GAL7 GAL10 Eukaryotic gene structure GAL1, GAL7, or GAL10 Basal transcription complex (RNAPII; TBP, other proteins) GAL4 binding GAL4 Transcription factor bound to DNA In a gal4 mutant, no GAL1, GAL7 or GAL10 protein is made under inducing conditions (galactose present). There is a another regulatory gene, GAL80 . In a gal80 deletion mutant, GAL1 GAL7 GAL10 GAL4 GAL80 (no Gal 80 is made) GAL1, GAL7 or GAL10 proteins are always made, even under non inducing conditions (no galactose present).
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