JAS_Garrett etal_2008_86_3315-3323_Promoter Bovine GH SNP assoc performance

JAS_Garrett etal_2008_86_3315-3323_Promoter Bovine GH SNP assoc performance

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A. J. Garrett, G. Rincon, J. F. Medrano, M. A. Elzo, G. A. Silver and M. G. Thomas polymorphism discovery in cattle and association with performance in Brangus bulls Promoter region of the bovine growth hormone receptor gene: Single nucleotide doi: 10.2527/jas.2008-0990 originally published online Aug 1, 2008; 2008.86:3315-3323. J Anim Sci http://jas.fass.org/cgi/content/full/86/12/3315 the World Wide Web at: The online version of this article, along with updated information and services, is located on www.asas.org at New Mexico State University on November 26, 2008. jas.fass.org Downloaded from
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ABSTRACT: Expression of the GH receptor (GHR) gene and its binding with GH is essential for growth and fat metabolism. A GT microsatellite exists in the promoter of bovine GHR segregating short (11 bp) and long (16 to 20 bp) allele sequences. To detect SNP and complete an association study of genotype to phenotype, we resequenced a 1,195-bp fragment of DNA including the GT microsatellite and exon 1A. Resequencing was completed in 48 familialy unrelated Holstein, Jersey, Brown Swiss, Simmental, Angus, Brahman, and Bran- gus cattle. Nine SNP were identified. Phylogeny analy- ses revealed minor distance (i.e., <5%) in DNA sequence among the 5 Bos taurus breeds; however, sequence from Brahman cattle averaged 27.4 ± 0.07% divergence from the Bos taurus breeds, whereas divergence of Brangus was intermediate. An association study of genotype to phenotype was completed with data from growing Brangus bulls (n = 553 from 96 sires) and data from 4 of the SNP flanking the GT microsatellite. These SNP were found to be in Hardy-Weinberg equilibrium and in phase based on linkage disequilibrium analyses (r 2 = 0.84 and D’= 0.92). An A/G tag SNP was identified (ss86273136) and was located in exon 1A, which began 88 bp downstream from the GT microsatellite. Minor allele frequency of the tag SNP was greater than 10%, and Mendelian segregation was verified in 3 generation pedigrees. The A allele was derived from Brahman, and the G allele was derived from Angus. This tag SNP genotype was a significant effect in analyses of rib fat data collected with ultrasound when bulls were ~365 d of age. Specifically, bulls of the GG genotype had 6.1% more ( P = 0.0204) rib fat than bulls of the AA and AG genotypes, respectively. Tag SNP (ss86273136), located in the promoter of GHR, appears to be associated with a measure of corporal fat in Bos taurus × Bos indicus composite cattle. Key words: bovine, Brangus, deoxyribonucleic acid, growth hormone receptor, single nucleotide polymorphism ©2008 American Society of Animal Science. All rights reserved. J. Anim. Sci. 2008. 86:3315–3323 doi:10.2527/jas.2008-0990 INTRODUCTION Growth hormone is involved in several metabolic and physiological processes (Etherton, 2004; Chagas et al., 2007). To elicit its effects, GH must bind its recep- tor ( GHR ; Edens and Talamantes, 1998; Zhu et al., 2001). Thus, DNA sequence variations in GHR may be beneficial for understanding how genotype(s) influ-
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JAS_Garrett etal_2008_86_3315-3323_Promoter Bovine GH SNP assoc performance

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