Histologylabmanual

Histologylabmanual - C O M P A R A T I V E H I S T O L O G...

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Unformatted text preview: C O M P A R A T I V E H I S T O L O G Y V A N B 6 0 2 H I S T O L O G Y L A B O R A T O R Y M A N U A L 2 0 1 0 D e p a r t m e n t o f A n i m a l B i o l o g y S c h o o l o f V e t e r i n a r y M e d i c i n e U n i v e r s i t y o f P e n n s y l v a n i a 3 8 0 0 S p r u c e S t r e e t P h i l a d e l p h i a , P A 1 9 1 0 4 2 LAB 1 EPITHELIUM # 2 - umbilical cord # 95 - thyroid gland # 65 - gallbladder # 52 - jejunum # 69 - esophagus/trachea # 80 - boar epididymis # 41 - esophagus # 36 - lip # 33 - planum nasolabiale # 75 - ureter # 76 - bladder # 83 - urethra # 91 - mammary gland # 93 - teat sinus or canal 1. Simple Squamous Epithelium . Slide 2 is a section of the umbilical cord . Hold the slide to the light and observe that there are up to 5 lumens present. One is the urachus , a functionless remnant of part of the duct of the allantois of the embryo. It extends from the bladder to the umbilicus. The other lumens are the umbilical arteries and veins . Examine these at this time. In general, the lumens of blood vessels and lymphatic vessels are lined by a single layer of flat epithelial cells known as endothelium . Endothelium is the type of simple squamous epithelium that lines vessels. Look at the endothelium of the large umbilical vessels as well as the many smaller vessels in the area. Only the nuclei, which bulge into the lumen, are visible. 2. Simple Cuboidal Epithelium . Slide 95 is a section of the thyroid gland . Thyroid follicles surround lumens that may be filled with a homogeneous colloid (dark pink in color). Note that each follicle is lined by a single layer of cells that are about as wide as they are tall. This epithelium is considered simple cuboidal . 3. Simple Columnar Epithelium . Slide 65 is a section of the wall of the gallbladder . Slide 52 is a section of the jejunum , a part of the small intestine. The epithelium lining the gallbladder and the jejunum consists of a single layer of cells whose height exceeds their width; therefore, this epithelium is considered simple columnar . Again, note that each epithelium lining surrounds a lumen. Some versions of slide 52 have large intestinal parasites that occupy the center of lumen. These are, of course, not a normal part of jejunal tissue and should be ignored for the time being. The epithelium in the intestine covers the villi, which are seen as finger- like projections into the lumen. Notice that the nuclei of the jejunal epithelium form a single row and that the cells are tall and thin. The lumenal, or apical side of the epithelium has a striated border , formed by microvilli on the apical domains of the epithelial cells. This is true for both the gallbladder and the small intestine. Other cell types may also be found in the epithelium of the jejunum, such as goblet cells. These will be discussed further in later labs. 4. Pseudostratified Columnar Epithelium . Pseudostratified columnar epithelium consists of basal cells and columnar cells. Both cell types rest on a basal lamina, but only the columnar cells reach the lumen. Since the nuclei of basal lamina, but only the columnar cells reach the lumen....
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Histologylabmanual - C O M P A R A T I V E H I S T O L O G...

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