immunology00426-0074 - Immunology,1965,9,61. The Functional...

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Unformatted text preview: Immunology,1965,9,61. The Functional Development oftheReticulo-Endothelial System II. THE HISTOLOGY OF BLOOD CLEARANCE BY THE FIXED MACROPHAGES OF FOETAL RATS P.C.READE* AND J.R. CASLEY-SMITHt MicrobiologyDepartmentandElectron MicroscopeUnit, DepartmentsofBotany, Microbiology and Zoology,University of Adelaide, SouthAustralia (Received5thOctober 1964) Summary. A histological study has been made ofthe distribution ofparticles injectedintravenouslyintofoetalrats.It was shownthattheorgandistributionwas similartothatobservedinadultratsand thatthe particles removedfromthecir- culatingblood were to befoundinsidethefixed macrophages. INTRODUCTION Numerous histological investigations of the cells and organs involved in clearing particles from the circulating blood ofnatal mammals have been reported at length sincethemiddleofthelastcentury.A variety of microorganisms and non-viable particles such ascolloidalmetals and particulatedyeshave been injected intosuch animals. The voracity with which certain fixed cells remove particulate matter from the blood by phagocytosishasbeen demonstrated. Ithasbeenshown repeatedlythattheliveriswell endowedwiththesephagocytesanditisthisorganwhichisconsideredtocontainmore of thesecellsofthereticuloendothelialsystem thanany other(Florey, 1961). SincetheearlydescriptionsbyworkerssuchasKupffer (1878, 1899)ithasbeenrecog- nizedthatthesefixedhepaticmacrophagesareinanidealanatomicalsituationforconstant monitoring of the circulating blood and clearing itofforeign particles. These early investigatorsworkingwiththetechnicallimitationsoftheirtimedescribedthesecellswith considerableaccuracy. Itisonlyinrecentyears thattheuse ofelectronmicroscopyhas corroboratedthesefindingsand added informationoffinedetail(Parks, 1956;Jezequel, 1962). Despitethelargevolume ofliteraturethatexistsconcerningthehistologyofphagocy- tosisbythecellsofthereticulo-endothelialsysteminnatalanimals,nopreviousreference can befoundtoacomprehensivestudyinfoetalmammals. Followingthedemonstrationthatvariousorgansoffoetalratswerecapableofcollecting injectedparticles and soclearingtheircirculatingbloodofforeignmaterial (Reade and Jenkin, 1965) itwas decided tostudy thisclearance by histological methods. Itwas the purpose ofthisstudy toinvestigate the reasons forclearance in foetalratsand hence to determinewhether thiswas due tomechanical trapping, surface adherence orphagocy- tosis. * NuffieldResearch Fellow, 1962-63. t SeniorLecturerinElectron-Microscopy. C IMMUN. 61 P.C.ReadeandJ.R.Casley-Smith Becauseoftheclearlydefinedway inwhich carboncanbedemonstratedinhistological sectionsand becauseitsdistributionover thetime periodstudiedappeared tobesimilar to thatofotherparticlesitwas usedinthisstudyasthemodelforthepatternofclearance ofintravenouslyinjectedparticlesfromthebloodoffoetalrats....
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immunology00426-0074 - Immunology,1965,9,61. The Functional...

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