Nerve Staining

Nerve Staining - HISTOLOGY LECTURE # 29 INTRODUCTION TO...

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Unformatted text preview: HISTOLOGY LECTURE # 29 INTRODUCTION TO SPECIAL STAINS TECHNIQUES: NERVE TISSUE STAINING Rationale : Special stain techniques are one of the major processes done in the histology laboratory. These techniques are performed to be evaluated with the diagnostics slides from H&Es. These stains are used as an aid and a diagnostic tool for a final diagnosis. Objective: Once completed this lecture, the student should be able to: a) Describe the different types of nerve tissue staining. b) Learn the various methods of stain demonstration. c) Learn the classification for Neurofibrils, axon, glial, astrocytes and myelin sheath staining. d) Learn the procedures and diagnostic tools for each stain. NERVE TISSUE Introduction Nerve Tissue Nerve tissue can be divided into two anatomical parts: Central Nervous System (CNS) Brain & Spinal cord Peripheral Nervous System (PNS) All other nervous system 1 2 In histology, nervous tissue consists of cells and cells processes and the various components of nerve tissues. These normally fall in three groups: Neuronal cells and processes Glial cells and processes Myelin sheath Neurons 1. Nissl Substances - basophilic material in the cytoplasm of the neuron 2. Nerve Cell Processes Dendrites & Axons Neuroglia 1. Oligodendroglia small cells that function in the CNS to produce & probably maintain, the myelin sheath surrounding many axons 2. Astrocytes stellate cells of two types: Protoplasmic, which occurs in the gray matter, and fibrous which occurs in the white matter 3. Microglia fixed phagocytic cells found throughout the brain and spinal cord 4. Ependymal cells true epithelial cells that line the ventricles and spinal canal, forms a barrier between the cerebrospinal fluid and nervous tissue Myelin 1. Is a complex, white, fatty, nonliving material containing protein, cholesterol, phospholipids, and cerebrosides. 2. It is largely loss during routine paraffin processing with only neurokeratin, a resistant proteolipid, left in the embedded block. 3. It is formed by oligodendroglia in the central nervous system and by Scwann cells in the peripheral nervous system. These are normally demonstrated by Luxol Fast Blue and Iron hematoxylin Nissl Substance: Cresyl Echt Violet Method (Luna, 1960) I. Purpose of the stain : Identification of neurons in tissue sections, or demonstration of loss of Nissl substances (chromatolysis). II. Principle of the stain : They are very basophilic and will stain sharply with basic aniline dyes. By varying the pH either Nissl substances can be stained by itself or nuclei and Nissl substances can be stained. III. Fixatives : 10% Neutral buffered formalin is preferred....
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This note was uploaded on 01/16/2012 for the course BI 200 taught by Professor Potter during the Fall '11 term at Montgomery College.

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Nerve Staining - HISTOLOGY LECTURE # 29 INTRODUCTION TO...

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