huang_lijuan - ALKALINE PHOSPHATASE SENSORS BASED ON...

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1 ALKALINE PHOSPHATASE SENSORS BASED ON AMPLIFIED QUENCHING OF CONJUGATED POLYELECTROLYTES By LIJUAN HUANG A THESIS PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE UNIVERSITY OF FLORIDA 2008
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2 © 2008 Lijuan Huang
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3 To my parents
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4 ACKNOWLEDGMENTS I would like to express my gratitude to all those who helped, encouraged and instructed me during my past years of MS studies in UF. Without them, I could not have completed this thesis. First of all, I would like to express my great appreciation to my supervisor, Dr. Kirk S. Schanze for his encouragement and support throughout this work. He guided me to this fantastic area of conjugated polymers and biosensors, which I barely knew before I joined his group. He encouraged me to learn new techniques and be an independent thinker. His enthusiasm for science and his kindness to people have a remarkable impact on my current studies and future careers. I also would like to thank the current and former members in the Schanze group for creating an excellent working environment. Special thanks are given to Dr. Yan Liu, who not only trained me to use the instruments, but also shared her experience and knowledge in the biosensor research. I have learnt a lot from her. I would like to thank Dr. Xiaoyong Zhao for synthesis of the wonderful conjugated polymers and discovered their sensing potentials. His work is the basis of the research described in this thesis. I would also like to thank Dr. Hui Jiang for his willingness to teach and help others. Whenever I have questions in the photochemistry, he is always there with answers. I give my thanks to Julia keller and Abigail Shelton for managing the orders for the group. I also want to thanks many other people, Dr. Kye-Young Kim, Johnathan Sommer, Dr. John Peak, Yongjun Li, Jarret Vella, Emine Demir, Eunkyung Ji, Seuong-Ho Lee, Zhuo Chen, Dongping Xie, Chen Liao, Dr. Richard Farley, Dr. Katsu Ogawa. My research and life in the lab become easier with their help and kindness. I am also thankful to Dr. Ben Smith and Dr. Linda Bloom for being my committee members.
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5 Finally, I am grateful to my parents. They have provided me the best conditions for my education and always teach me to be an optimistic person. I would not be able to be anywhere without their unselfish love and support. I want to thank my boyfriend for always being there for me in the past years, especially in hard times.
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6 TABLE OF CONTENTS page ACKNOWLEDGMENTS . .............................................................................................................. 4 LIST OF FIGURES . ........................................................................................................................ 8 INTRODUCTION . ........................................................................................................................ 12 Conjugated polyelectrolytes . .................................................................................................. 12 Fluorescence Quenching . ........................................................................................................ 15 Amplified Quenching of Conjugated Polyelectrolytes . .......................................................... 18 Aggregation of CPEs . ............................................................................................................. 22 Applications of conjugate polyelectrolytes as sensors . .......................................................... 24 Quencher induced quenching-unquenching mechanism . ................................................ 25 Chain conformation perturbation mechanism . ................................................................ 27 Fluorescence resonance energy transfer (FRET) mechanism . ........................................ 29 Alkaline phosphatase assay . ................................................................................................... 34 Alkaline phosphatase sensors using CHEMILUMINESCENT CONJUGATED POLYMER . ....37 Introduction±. ............................................................................................................................ 37 Results and Discussion . .......................................................................................................... 40
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This note was uploaded on 01/15/2012 for the course CHM 3610 taught by Professor Scott during the Fall '09 term at University of Florida.

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huang_lijuan - ALKALINE PHOSPHATASE SENSORS BASED ON...

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