121lab03 - Laboratory 3 - Enzymes Beano, Enzymes to the...

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1 Laboratory 3 - Enzymes Beano ® , Enzymes to the Rescue: In Vitro Gas Suppression 1 This lab is adapted from Shoestring Biotechnology (in press) and is used with permission from Kathy Frame of the National Association of Biology Teachers. Learning Objectives Students will be able to: Form a hypothesis for the experiment to be performed. Test for the effectiveness of enzymes in reducing gas production. Understand how enzymes are used in digestion. Summarize how the body digests complex carbohydrates. After reading these guidelines, students should be able to answer the following questions: What is Beano ® ? How does the body digest oligosaccharides? What are the products of digestion of oligosaccharides? Why will glucose levels be measured and how? How much gas does our body produce daily? Synopsis Beano ® is produced through the modern fermentation techniques of the biotechnology industry. This enzyme can be applied to food before eating to help prevent gas production in the human colon. Your group will design an experiment to test the activity or effectiveness of the Beano ® enzyme, alpha – ( β -) galactosidase. Background Information Beans are seeds that contain complex carbohydrates called oligosaccharides. The plant hormone gibberillin initiates the release of the enzymes amylase and β - galactosidase to act on the oligosaccharides to produce the energy for seed germination. However, when humans consume beans, we lack the β -galactosidase 1 Template Developer: Judy Brown, Edison Career Center, 12501 Dalewood Drive, Wheaton, MD 20906. Sections have been adapted with permission from Beano ® Lab for NABT 1994 conceived by Dr. Lee Williams, Alamance Community College, NC and modified by Britt Hammond, Eastern Guilford High School, NC. Further development and editing: Lana Hays, Simon Kenton High School, 11132 Madison Pike, Independence, KY, 41051.
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2 enzyme. The oligosacharrides cannot be broken down with the repertoire of human enzymes. Enzymes are specific for the substrate that they act upon. If the enzyme is lacking, the substrate cannot be broken down. When we consume a meal of beans, most individuals will experience pressure soon after in the abdominal area. To understand why this occurs, we have to understand how beans are digested in our bodies. As the beans enter the human digestive tract, enzymes present in the stomach begin to break them down, but the majority of digestion occurs with enzymes in the small intestine. Any material that is not digested by these enzymes becomes part of the fiber that leaves the small intestine undigested and enters the large intestine or colon. In this case, it is the oligosaccharides of the bean. These undigested bean carbohydrates enter the colon and are exposed to the microbes that live within our intestine. While humans do not have the enzyme β -galactosidase, the microbes that reside within our intestines do. They break down the oligosaccharides to produce simple sugars,
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This note was uploaded on 01/16/2012 for the course NUTR 121 taught by Professor Heathergraham during the Fall '11 term at Truckee Meadows Community College.

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121lab03 - Laboratory 3 - Enzymes Beano, Enzymes to the...

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