This is the burst phase whichproduces the colored product..This acyl-enzyme intermediate complex is then hydrolyzed to release thecarboxylic acid component of the substrate and regenerate the free enzymeas shown below.This is the steady-statephase, which takes longerfor the enzyme to be released.
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•Catalytic triadS 195 is H-bonded to the imidazolering of H 57. The –NH group of theimidazole ring of H 57 is, in turn,H-bonded to the carboxylate groupof D 102.The arrangement of these 3 residuesis known as the catalytic triad.The H 57 positions the S 195 to polarizeits hydroxyl group so it can be deproton-ated. The D 102 helps orient H 57 andmake it a better proton acceptor.