FA2011lecture15

FA2011lecture15 - Bacterial genome chromsomal DNA containing essential genes plus one or more plasmids encoding specialized features of certain

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Unformatted text preview: Bacterial genome: chromsomal DNA containing essential genes plus one or more plasmids encoding specialized features of certain bacteria, for example: • Pathogenic properties of bacteria causing anthrax, plague, dysentery and tetanus due to toxin/virulence genes borne on plasmids • Plasmid genes confer resistance to antibiotics (MDR=multidrug resistant bacteria, MRSA=methicillin- resistant S. aureus ) • Ability of some bacterial species to x nitrogen in symbiotic association with plants is due to nitrogen- xing proteins encoded by plasmid genes • Ability of some bacteria to degrade environmental pollutants (e.g. oil) due to genes on plasmids E. coli + biotin- biotin bio + bio- + + +- + arginine - arginine arg + + + arg- +- + galactose + lactose gal + + + gal-- + + strep - strep strp s- + strp r + + geno- type added to medium Examples of E. coli mutant phenotypes Conjugation in E. coli F + F- pili Transfer of F plasmid from F + to F- E. coli via conjugation and rolling circle replication not drawn to scale (really 2% of E. coli chromosome) Hfr =...
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This note was uploaded on 01/16/2012 for the course BICD 100 taught by Professor Nehring during the Fall '08 term at UCSD.

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FA2011lecture15 - Bacterial genome chromsomal DNA containing essential genes plus one or more plasmids encoding specialized features of certain

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