Review Session 5 Key

Review Session 5 Key - Sun. Oct. 30 review session for...

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Sun. Oct. 30 review session for BICD100 FA2011 Topics: mapping with DNA markers, LOD scores, bacterial genetics, 1.
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2. An outbreak of food poisoning has been tracked down to a pathogenic strain of E. coli, which makes people sick because it has a gene encoding a toxic protein. Unlike most bacterial toxin genes, this one is unusual in being encoded by a gene on the E. coli chromosome rather than a plasmid, and a microbiologist wants to identify and study this gene. F plasmids are introduced into the pathogenic strain via conjugation with F + bacteria, and four different Hfr strains are isolated (Hfr1 – Hfr4). Each of the pathogenic Hfr strains, which are strep S and wild-type for each of the other genes shown on the map of the E. coli chromosome shown below, is mated with an F - strep R arg - pro - phe - cys - met - strain. Time of entry mapping experiments yield the following results when disrupted mating pairs are plated out on streptoymycin: Hfr1 time gene Hfr2 time gene Hfr3 time gene
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Review Session 5 Key - Sun. Oct. 30 review session for...

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