UC Davis Lab Skill Sheet

UC Davis Lab Skill Sheet - LABORATORY SKILLS SHEET Use Tab...

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Unformatted text preview: LABORATORY SKILLS SHEET Use Tab key to move between fields- shift/tab to go backwards NAME:       DATE :       Use the rating scale below to indicate your level of proficiency on tasks which you are capable of performing or have knowledge to perform in a working environment. Leave blank if you are not capable of performing or lack knowledge to perform the task. 1. HIGHLY SKILLED — have very recent experience 2. WELL SKILLED — have performed within two years 3. SOME SKILL — need retraining 4. IN-DEPTH KNOWLEDGE, BUT LACK SKILL TO PERFORM — need training ANIMAL CARE Handling and restraining:      Monkeys      Mice and Rats      Cats      Rabbits      Invertebrates      Dogs      Sheep      Poultry      Swine      Feeding      Cleaning Cages      Horses      Dairy Cattle      Beef Cattle      Goats      Breeding      Assisting in veterinary procedures ANIMAL SURGERY      Preparation for sterile surgery      Assist in sterile procedure      Endotrachial intubation and use of ventilators      Administration of anesthesia: TYPE:            Venous      Intraperitoneal      Subcutaneous      Tumor implantation into small animals      Animal Autopsy      Microsurgery      Catheterization and infusion of small animal arteries      Collection of blood      Post-surgery care MICROBIOLOGY — BACTERIOLOGY      Maintenance of cultures      Isolation and purification Determination and concentration by:      Direct colony counting      Antibody titer      Direct microscope observation      Infectivity assay      Inoculation      Media preparation      Preparation and assay of bacteriophage      Plate pouring MICROBIOLOGY/VIROLOGY      Fluorescent stains      Reverse transcriptase      Serological tests      Hemagglutination inhibition      Complement fixation      Neutralization      RIA      Virus identification      Cytopathic effect      Hemadsorption      Interferon production      Other:      _________________________ Revised 08/2001 TISSUE CULTURE      Maintenance and division of continuous lines      Establish primary cell lines      Screening for mycoplasma contamination      Isolation of cell mutants      Cell fusion      Cell hybridization      Staining cells      Cell cloning      P3 lab precautions      Protein and DNA synthesis assays GENETICS      Mutagenesis      Mutation tests      Transduction      Conjugation      Maintenance and breeding genetic stock      Transformation      Extraction of plasmid DNA      Isolation of plasmid DNA      Antibiotic resistance tests BIOCHEMISTRY Cell fractionation:      Homogenization      Sonication      Differential centrifugation Purification/characterization of:      Enzymes      Proteins      Phosphate      RNA      Nucleic acids      Glycoproteins      Other:      ______________________ Gradients:      Density      Equilibrium      Ficoll      Other:      _________________________ Assays:      Enzyme      Protein      RNA      DNA      Lipids      Phosphates      Receptor binding      Competitive binding      Other:      _________________________ Electrophoresis:      Immuno      Acrylamide gel      Columns      Paper      Agarose      Labs      Isoelectric focusing      2-dimensional gels Chromotography:      Paper      Thin layer      Affinity      Column      High pressure liquid      Ion exchange      Gas-liquid      Immuno-precipitin techniques In-vitro synthesis of:      Peptides      DNA      RNA      DNA shearing ELECTRON MICROSCOPY      Staining      Embedding      Sectioning      Operation of TEM      SEM      Grid preparation      Measuring molecules      Shadowing      Freeze fracture      Diffusion technique of DNA spread GENERAL LABORATORY TECHNIQUES      Pipetting      Weighing      Sterilizing      Titration      Filtering techniques      Glassware washing      Autoclave      Steam and gas      Sterile technique      Reagent and buffer preparation IMMUNOLOGY      Plaque assay for antibody forming cells      Hemagglutination and hemolysis assay      Fluorescent antibody technique      Spectrofluorometry      Lyophilization      Microhemagglutination      Virus isolation      Antibody titration      HLA typing      E-rosette assay      Plate binding assay      Making antibodies in animals      Making monoclonal antibodies HISTOLOGY      Perfusion of animals      Fixation of tissue Processing and embedding in:      Paraffin      Celloidin      Gelatin Paraffin sectioning of:      Biopsy tissue      Autopsy tissue      Animal tissue      Neurologic tissue      Serial sectioning      Decalcification Staining methods:      Routine hematoxylin and eosin      Special stains      Metallic impregnation      Histochemical      Autography of tissue sections Microtomes:      Rotary      Sliding      Cryostat PHYSIOLOGY      Preparation of chronic animals      Stereotaxic implantation of electrodes/cannulae      Preparation of acute animals      EEG recording      EKG recording Electrode fabrication:      Intracellular      Extracellular      Preparation of brain slices for in vitro recording RADIOBIOLOGICAL      Bioassays of radioactive isotopes      Radioimmunoassays of steroids      Radioisotope handling      Irradiation of tumors in vitro      Radioactive labeling      Other:      _________________________ Revised 08/2001 BIOCHEMICAL INSTRUMENTS      Autoanalyzer      Microburet      Fluorometer      pH meter Spectrophotometers:      Gamma      UV      Visible      Colorimeter      Recording      Atomic absorption      ESR      Scintillation counter      Fraction collectors      Freeze dryers      Refractometer Centrifuges:      Standard      High speed      Model L Preparative Ultracentrifuge      Model E Analytical Ultracentrifuge OTHER INSTRUMENTS      Incubator      Darkroom equipment      Oscillograph      Volumetric glassware      Blood gas analyzer      Hemocytometer      Plaque counter      Densitometer      Micropipetting equipment      Automatic dispenser      Particle counter      Gamma counter      Microphotographic equipment ELECTRONICS EQUIPMENT      Design      Construction      Troubleshooting      Maintenance      Calibrate DATA PROCESSING      Systems programming      Operating computer system      High level programming on large computers      Desk top calculators      Work with Programmer      Numerical calculations involving chemical stoichiometry      Statistics      Database management COMPUTER LANGUAGES      FORTRAN      BASIC      ALGOL      Assembly      Other:      ______________________ PHOTOGRAPHY      Black/white processing      Black/white printing      Color processing      Color printing      Line negative printing      Continuous tone printing      Internegative printing/processing      Transparencies AGRICULTURE      Management      Marketing      Crop production      Field plot research      Greenhouse      Soil science      Plant science      Plant breeding      Bacterial virus identification      Test plot design and layout      Specific anthropod nematode      Storage techniques ...
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