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12A Yeast Respiration (CO2)

12A Yeast Respiration (CO2) - Experiment 12A Respiration of...

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Biology with Calculators 12A - 1 Experiment 12A Respiration of Sugars by Yeast Yeast are able to metabolize some foods, but not others. In order for an organism to make use of a potential source of food, it must be capable of transporting the food into its cells. It must also have the proper enzymes capable of breaking the food’s chemical bonds in a useful way. Sugars are vital to all living organisms. Yeast are capable of using some, but not all sugars as a food source. Yeast can metabolize sugar in two ways, aerobically, with the aid of oxygen, or anaerobically , without oxygen. In this lab, you will try to determine whether yeast are capable of metabolizing a variety of sugars. When yeast respire aerobically, oxygen gas is consumed and carbon dioxide, CO 2 , is produced. You will use a CO 2 Gas Sensor to monitor the production of carbon dioxide as yeast respire using different sugars. The four sugars that will be tested are glucose (blood sugar), sucrose (table sugar), fructose (fruit sugar), and lactose (milk sugar). 1. C O G a s S e n s o r 2 Figure 1 MATERIALS LabPro or CBL 2 interface 600-mL beaker (for water bath) TI Graphing Calculator Beral pipettes DataMate program hot and cold water Vernier CO 2 Gas Sensor thermometer 250-mL respiration chamber four 10 X 100 mm test tube 5% glucose, sucrose, lactose, and fructose sugar solution yeast suspension Graphical Analysis (optional)
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12A - 2 Biology with Calculators Experiment 12A PROCEDURE 1. Prepare a water bath for the yeast. A water bath is simply a large beaker of water at a certain temperature. This ensures that the yeast will remain at a constant and controlled
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