Conclusion Plant Pigments

Conclusion Plant Pigments - Conclusion Isolation and...

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Conclusion Isolation and Purification of Plant Pigments The purpose of the experiment was to properly separate three pigments from plant extract. The procedure done in lab involved setting up a microsale column and preparing it with 6 mL of hexane and 6 mL of alumina powder. The hexane was poured in first, and the solvent was added slowly in order to ensure no channels or blockages were created. We then created a sand layer between the hexane and alumina powder to ensure they would not interact act. Once the column was set up and packed correctly and hexane was drained to sand level, 1.0 mL of plant pigment extract was added. The plant pigment contains three separate pigments each varying in polarity. The pigments are separated based on polarity. In order to drain the least polar pigment (B carotene) we used a 90:10 hexane, which is, not vary polar. In order to pull out chlorophyll a which is more polar, it was necessary to increate the polarity of the hexane. The b carotene elution used 90:10
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This note was uploaded on 01/18/2012 for the course CHEMISTRY 308 taught by Professor Jonscaggs during the Fall '11 term at Boise State.

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